Chemical quenching of singlet oxygen by plastoquinols and their oxidation products in Arabidopsis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F18%3A73590093" target="_blank" >RIV/61989592:15310/18:73590093 - isvavai.cz</a>

Result on the web

<a href="https://onlinelibrary.wiley.com/doi/full/10.1111/tpj.13993" target="_blank" >https://onlinelibrary.wiley.com/doi/full/10.1111/tpj.13993</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/tpj.13993" target="_blank" >10.1111/tpj.13993</a>

Result language

angličtina

Original language name

Chemical quenching of singlet oxygen by plastoquinols and their oxidation products in Arabidopsis

Original language description

Prenylquinols (tocochromanols and plastoquinols) serve as efficient physical and chemical quenchers of singlet oxygen (1O2) formed during high light stress in higher plants. Although quenching of 1O2 by prenylquinols has been previously studied, direct evidence for chemical quenching of 1O2 by plastoquinols and their oxidation products is limited in vivo. In the present study, the role of plastoquinol-9 (PQH2-9) in chemical quenching of 1O2 was studied in Arabidopsis thaliana lines overexpressing the SOLANESYL DIPHOSPHATE SYNTHASE 1 gene (SPS1oex) involved in PQH2-9 and plastochromanol-8 biosynthesis. In this work, direct evidence for chemical quenching of 1O2 by plastoquinols and their oxidation products is presented, which is obtained by microscopic techniques in vivo. Chemical quenching of 1O2 was associated with consumption of PQH2-9 and formation of its various oxidized forms. Oxidation of PQH2-9 by 1O2 leads to plastoquinone-9 (PQ-9), which is subsequently oxidized to hydroxyplastoquinone-9 [PQ(OH)-9]. We provide here evidence that oxidation of PQ(OH)-9 by 1O2 results in the formation of trihydroxyplastoquinone-9 [PQ(OH)3-9]. It is concluded here that PQH2-9 serves as an efficient 1O2 chemical quencher in Arabidopsis, and PQ(OH)3-9 can be considered as a natural product of 1O2 reaction with PQ(OH)-9. The understanding of the mechanisms underlying 1O2 chemical quenching provides information on the role of plastoquinols and their oxidation products in the response of plants to photooxidative stress.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10610 - Biophysics

Project

<a href="/en/project/LO1204" target="_blank" >LO1204: Sustainable development of research in the Centre of the Region Haná</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

PLANT JOURNAL

ISSN

0960-7412

e-ISSN

—

Volume of the periodical

95

Issue of the periodical within the volume

5

Country of publishing house

GB - UNITED KINGDOM

Number of pages

14

Pages from-to

"848–861"

UT code for WoS article

000441759400009

EID of the result in the Scopus database

2-s2.0-85051487358