Membrane depolarization and aberrant lipid distributions in the neonatal rat brain following hypoxic-ischaemic insult

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F18%3A73591527" target="_blank" >RIV/61989592:15310/18:73591527 - isvavai.cz</a>

Alternative codes found

RIV/61388971:_____/18:00496976

Result on the web

<a href="https://www.nature.com/articles/s41598-018-25088-2" target="_blank" >https://www.nature.com/articles/s41598-018-25088-2</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41598-018-25088-2" target="_blank" >10.1038/s41598-018-25088-2</a>

Result language

angličtina

Original language name

Membrane depolarization and aberrant lipid distributions in the neonatal rat brain following hypoxic-ischaemic insult

Original language description

Neonatal hypoxic-ischaemic (HI) encephalopathy is among the most serious complications in neonatology. In the present study, we studied the immediate (0 hour), subacute (36 hours) and late (144 hours) responses of the neonatal brain to experimental HI insult in laboratory rats. At the striatal level, the mass spectrometry imaging revealed an aberrant plasma membrane distribution of Na+/K+ ions in the oedema-affected areas. The failure of the Na+/K+ gradients was also apparent in the magnetic resonance imaging measurements, demonstrating intracellular water accumulation during the acute phase of the HI insult. During the subacute phase, compared with the control brains, an incipient accumulation of an array of N-acylphosphatidylethanolamine (NAPE) molecules was detected in the HI-affected brains, and both the cytotoxic and vasogenic types of oedema were detected. In the severely affected brain areas, abnormal distributions of the monosialogangliosides GM2 and GM3 were observed in two-thirds of the animals exposed to the insult. During the late stage, a partial restoration of the brain tissue was observed in most rats in both the in vivo and ex vivo studies. These specific molecular changes may be further utilized in neonatology practice in proposing and testing novel therapeutic strategies for the treatment of neonatal HI encephalopathy.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10406 - Analytical chemistry

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Scientific Reports

ISSN

2045-2322

e-ISSN

—

Volume of the periodical

8

Issue of the periodical within the volume

MAY

Country of publishing house

GB - UNITED KINGDOM

Number of pages

11

Pages from-to

"6952-1"-"6952-11"

UT code for WoS article

000431291500004

EID of the result in the Scopus database

2-s2.0-85046631066