Does the Pollen Diet Influence the Production and Expression of Antimicrobial Peptides in Individual Honey Bees?

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F18%3A73591830" target="_blank" >RIV/61989592:15310/18:73591830 - isvavai.cz</a>

Result on the web

<a href="https://www.mdpi.com/2075-4450/9/3/79/htm" target="_blank" >https://www.mdpi.com/2075-4450/9/3/79/htm</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/insects9030079" target="_blank" >10.3390/insects9030079</a>

Result language

angličtina

Original language name

Does the Pollen Diet Influence the Production and Expression of Antimicrobial Peptides in Individual Honey Bees?

Original language description

We investigated the importance of protein nutrition for honey bee immunity. Different protein diets (monofloral pollen of Helianthus spp., Sinapis spp., Asparagus spp., Castanea spp., a mixture of the four different pollen and the pollen substitute Feedbee(TM)) were fed to honey bees in cages ad libitum. After 18 days of feeding, apidaecin 1 isoforms concentration in the thorax were measured using nanoflow liquid chromatography coupled with mass spectrometry. Expression levels of genes, coding for apidaecins and abaecin in the abdomen were determined using quantitative PCR. The results indicate that protein-containing nutrition in adult worker honey bees can trigger certain metabolic responses. Bees without dietary protein showed lower apidaecin 1 isoforms concentrations. The significantly lowest concentration of apidaecin 1 isoforms was found in the group that was fed no pollen diet when compared to Asparagus, Castanea, Helianthus, and Sinapis pollen or the pollen supplement FeedBee(TM). Expression levels of the respective genes were also affected by the protein diets and different expression levels of these two antimicrobial peptides were found. Positive correlation between concentration and gene expression of apidaecins was found. The significance of feeding bees with different protein diets, as well as the importance of pollen nutrition for honey bee immunity is demonstrated.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10616 - Entomology

Project

<a href="/en/project/LO1204" target="_blank" >LO1204: Sustainable development of research in the Centre of the Region Haná</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Insects

ISSN

2075-4450

e-ISSN

—

Volume of the periodical

9

Issue of the periodical within the volume

3

Country of publishing house

CH - SWITZERLAND

Number of pages

12

Pages from-to

"79-1"-"79-12"

UT code for WoS article

000448335000007

EID of the result in the Scopus database

2-s2.0-85050525117