Chloroplasts preferentially take up ferric–citrate over iron–nicotianamine complexes in Brassica napus

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F19%3A73590207" target="_blank" >RIV/61989592:15310/19:73590207 - isvavai.cz</a>

Result on the web

<a href="https://link.springer.com/article/10.1007%2Fs00425-018-3037-0" target="_blank" >https://link.springer.com/article/10.1007%2Fs00425-018-3037-0</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00425-018-3037-0" target="_blank" >10.1007/s00425-018-3037-0</a>

Result language

angličtina

Original language name

Chloroplasts preferentially take up ferric–citrate over iron–nicotianamine complexes in Brassica napus

Original language description

Fe uptake machinery of chloroplasts prefers to utilise Fe(III)–citrate over Fe–nicotianamine complexes. Iron uptake in chloroplasts is a process of prime importance. Although a few members of their iron transport machinery were identified, the substrate preference of the system is still unknown. Intact chloroplasts of oilseed rape (Brassica napus) were purified and subjected to iron uptake studies using natural and artificial iron complexes. Fe–nicotianamine (NA) complexes were characterised by 5 K, 5 T Mössbauer spectrometry. Expression of components of the chloroplast Fe uptake machinery was also studied. Fe(III)–NA contained a minor paramagnetic Fe(II) component (ca. 9%), a paramagnetic Fe(III) component exhibiting dimeric or oligomeric structure (ca. 20%), and a Fe(III) complex, likely being a monomeric structure, which undergoes slow electronic relaxation at 5 K (ca. 61%). Fe(II)–NA contained more than one similar chemical Fe(II) environment with no sign of Fe(III) components. Chloroplasts preferred Fe(III)–citrate compared to Fe(III)–NA and Fe(II)–NA, but also to Fe(III)–EDTA and Fe(III)–o,o′EDDHA, and the Km value was lower for Fe(III)-citrate than for the Fe–NA complexes. Only the uptake of Fe(III)–citrate was light-dependent. Regarding the components of the chloroplast Fe uptake system, only genes of the reduction-based Fe uptake system showed high expression. Chloroplasts more effectively utilize Fe(III)–citrate, but hardly Fe–NA complexes in Fe uptake.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10611 - Plant sciences, botany

Project

<a href="/en/project/LO1305" target="_blank" >LO1305: Development of the center of advanced technologies and materials</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

PLANTA

ISSN

0032-0935

e-ISSN

—

Volume of the periodical

249

Issue of the periodical within the volume

3

Country of publishing house

DE - GERMANY

Number of pages

13

Pages from-to

751-763

UT code for WoS article

000459488700010

EID of the result in the Scopus database

2-s2.0-85055967624