Enzyme self-assembly on naked iron oxide nanoparticles for aminoaldehyde biosensing
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F19%3A73595180" target="_blank" >RIV/61989592:15310/19:73595180 - isvavai.cz</a>
Result on the web
<a href="https://link.springer.com/article/10.1007/s00726-019-02704-7" target="_blank" >https://link.springer.com/article/10.1007/s00726-019-02704-7</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00726-019-02704-7" target="_blank" >10.1007/s00726-019-02704-7</a>
Alternative languages
Result language
angličtina
Original language name
Enzyme self-assembly on naked iron oxide nanoparticles for aminoaldehyde biosensing
Original language description
The preservation of enzymatic activity is a fundamental requirement for exploiting hybrid nano-bio-conjugates, and the control over protein-nanoparticle interactions, leading to stable and catalytically active hybrids, represents the key for designing new biosensing platforms. In this scenario, surface active maghemite nanoparticles (SAMNs) represent a new class of naked magnetic nanoparticles, displaying peculiar electrocatalytic features and the ability to selectively bind proteins. Recombinant aminoaldehyde dehydrogenase from tomato (SlAMADH1) was used as a model protein, and successfully immobilized by self-assembly on the surface of naked SAMNs, where its enzymatic activity resulted preserved for more than 6months. The hybrid nanomaterial (SAMN@SlAMADH1) was characterized by UV-Vis spectroscopy, mass spectrometry, and TEM microscopy, and applied for the development of a biosensor for the determination of aminoaldehydes in alcoholic beverages. Measurements were carried out in a low volume electrochemical flow cell comprising a SAMN modified carbon paste electrode for the coulometric determination of the NADH produced during the enzymatic catalysis. The present findings, besides representing the first example of an electrochemical biosensor for aminoaldehydes in an alcoholic matrix, open the door to the use of immobilized enzymes on naked metal oxides nanomaterials for biosensing.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/LO1204" target="_blank" >LO1204: Sustainable development of research in the Centre of the Region Haná</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Amino Acids
ISSN
0939-4451
e-ISSN
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Volume of the periodical
51
Issue of the periodical within the volume
4
Country of publishing house
DE - GERMANY
Number of pages
12
Pages from-to
679-690
UT code for WoS article
000463590400011
EID of the result in the Scopus database
2-s2.0-85061179892