Occurrence and biosynthesis of cytokinins in poplar
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F19%3AN0000001" target="_blank" >RIV/61989592:15310/19:N0000001 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15310/19:73597029 RIV/00027006:_____/19:00005351
Result on the web
<a href="https://link.springer.com/article/10.1007/s00425-019-03152-z" target="_blank" >https://link.springer.com/article/10.1007/s00425-019-03152-z</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00425-019-03152-z" target="_blank" >10.1007/s00425-019-03152-z</a>
Alternative languages
Result language
angličtina
Original language name
Occurrence and biosynthesis of cytokinins in poplar
Original language description
Cytokinins are phytohormones with a fundamental role in the regulation of plant growth and development. They occur naturally either as isoprenoid or aromatic derivatives, but the latter are quite rare and less studied. Here, the spatial expression of all nine isopentenyl transferase genes of Populus × canadensis cv. Robusta (PcIPTs) as analyzed by RT-qPCR revealed a tissue preference and strong differences in expression levels for the different adenylate and tRNA PcIPTs. Together with their phylogeny, this result suggests a functional diversification for the different PcIPT proteins. Additionally, the majority of PcIPT genes were cloned and expressed in Arabidopsis thaliana under an inducible promoter. The cytokinin levels measured in the Arabidopsis-overexpressing lines as well as their phenotype indicate that the studied adenylate and tRNA PcIPT proteins are functional in vivo and thus will contribute to the cytokinin pool in poplar. We screened the cytokinin content in leaves of 12 Populus species by ultra-high performance-tandem mass spectrometry (UHPLC-MS/MS) and discovered that the capacity to produce not only isoprenoid, but also aromatic cytokinins is widespread amongst the Populus accessions studied. Important for future studies is that the levels of aromatic cytokinins transiently increase after daybreak and are much higher in older plants.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
<a href="/en/project/GA15-16888S" target="_blank" >GA15-16888S: Aromatic and isoprenoid cytokinins in poplar: biosynthesis and perception</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
PLANTA
ISSN
0032-0935
e-ISSN
1432-2048
Volume of the periodical
250
Issue of the periodical within the volume
1
Country of publishing house
DE - GERMANY
Number of pages
16
Pages from-to
229-244
UT code for WoS article
000471058300017
EID of the result in the Scopus database
2-s2.0-85064244769