Roles for ALDH10 enzymes in gamma-butyrobetaine synthesis, seed development, germination, and salt tolerance in Arabidopsis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F20%3A73603612" target="_blank" >RIV/61989592:15310/20:73603612 - isvavai.cz</a>
Result on the web
<a href="https://academic.oup.com/jxb/article/71/22/7088/5897428" target="_blank" >https://academic.oup.com/jxb/article/71/22/7088/5897428</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/jxb/eraa394" target="_blank" >10.1093/jxb/eraa394</a>
Alternative languages
Result language
angličtina
Original language name
Roles for ALDH10 enzymes in gamma-butyrobetaine synthesis, seed development, germination, and salt tolerance in Arabidopsis
Original language description
Plant genomes generally contain two aldehyde dehydrogenase 10 (ALDH10) genes, which encode NAD+-dependent enzymes. These oxidize various aminoaldehydes that are produced by the catabolism of amino acids and polyamines. ALDH10s are closely related to the animal and fungal trimethylaminobutyraldehyde dehydrogenases (TMABADHs) that are involved in the synthesis of γ-butyrobetaine, the precursor of carnitine. Here, we explore the ability of the Arabidopsis thaliana proteins AtALDH10A8 and AtALDH10A9 to oxidize aminoaldehydes. We demonstrate that these enzymes display high TMABADH activities in vitro. Moreover, they can complement the Candida albicans tmabadhΔ/Δnull mutant. These findings illustrate the link between AtALDH10A8 and AtALDH10A9 and γ-butyrobetaine synthesis. An analysis of single and double knockout Arabidopsis mutant lines revealed that the double mutants had reduced γ-butyrobetaine levels. However, there were no changes in the carnitine contents of these mutants. The double mu-tants were more sensitive to salt stress. In addition, the siliques of the double mutants had a significant proportion of seeds that failed to mature. The mature seeds contained higher amounts of triacylglycerol, facilitating accelerated germination. Taken together, these results show that ALDH10 enzymes are involved in γ-butyrobetaine synthesis. Furthermore, γ-butyrobetaine fulfils a range of physiological roles in addition to those related to carnitine biosynthesis.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
JOURNAL OF EXPERIMENTAL BOTANY
ISSN
0022-0957
e-ISSN
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Volume of the periodical
71
Issue of the periodical within the volume
22
Country of publishing house
GB - UNITED KINGDOM
Number of pages
15
Pages from-to
7088-7102
UT code for WoS article
000608422900024
EID of the result in the Scopus database
2-s2.0-85098802394