Stereoisomer separation of flavanones and flavanone-7-O-glycosides by means of nanoliquid chromatography employing derivatized beta-cyclodextrins as mobile-phase additive
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F20%3A73603970" target="_blank" >RIV/61989592:15310/20:73603970 - isvavai.cz</a>
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/full/10.1002/jssc.202000268" target="_blank" >https://onlinelibrary.wiley.com/doi/full/10.1002/jssc.202000268</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/jssc.202000268" target="_blank" >10.1002/jssc.202000268</a>
Alternative languages
Result language
angličtina
Original language name
Stereoisomer separation of flavanones and flavanone-7-O-glycosides by means of nanoliquid chromatography employing derivatized beta-cyclodextrins as mobile-phase additive
Original language description
A nanoliquid chromatographic method for the stereoisomer separation of some flavanone aglycones and 7-O-glycosides has been proposed employing a C18 capillary column and a chiral mobile-phase additive such as cyclodextrin. The chiral separation of eriodictyol, naringenin, and hesperitin was obtained by addition of carboxymethyl-beta-cyclodextrin to the mobile phase, whereas eriocitrin, naringin, narirutin, and hesperidin diastereoisomers were resolved by using sulfobutyl ether-beta-cyclodextrin. The influence of the composition of the mobile phase, the length of the capillary column, and the flow rate on the chiral recognition were investigated. At optimum conditions, baseline separation for the selected aglycones and glycosylated forms were achieved with a mobile phase consisting of 50 mM sodium acetate buffer pH 3 and 30% methanol containing 20 mM of carboxymethyl-beta-cyclodextrin and 10 mM of sulfobutyl ether-beta-cyclodextrin, respectively. Precision, linearity, and sensitivity of the method were tested. Limits of detection and quantification for the studied flavanone glycosides were in the range 1.3-2.5 and 7.5-12.5 mu g/mL, respectively. The method was used for the determination of the diastereomeric composition of the flavanone-7-O-glycosides inCitrusjuices after solid-phase extraction procedure.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/EF16_019%2F0000754" target="_blank" >EF16_019/0000754: Nanotechnologies for Future</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
JOURNAL OF SEPARATION SCIENCE
ISSN
1615-9306
e-ISSN
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Volume of the periodical
43
Issue of the periodical within the volume
17
Country of publishing house
DE - GERMANY
Number of pages
9
Pages from-to
3382-3390
UT code for WoS article
000557665400001
EID of the result in the Scopus database
2-s2.0-85089159720