Signal enhancement in desorption nanoelectrospray ionization by custom-made inlet with pressure regulation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F20%3A73604001" target="_blank" >RIV/61989592:15310/20:73604001 - isvavai.cz</a>
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/full/10.1002/jms.4642" target="_blank" >https://onlinelibrary.wiley.com/doi/full/10.1002/jms.4642</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/jms.4642" target="_blank" >10.1002/jms.4642</a>
Alternative languages
Result language
angličtina
Original language name
Signal enhancement in desorption nanoelectrospray ionization by custom-made inlet with pressure regulation
Original language description
The efficiency of desorption/ionization becomes more critical as the sampled surface area decreases. Desorption electrospray and desorption nanoelectrospray belong to ambient ionizations and enable direct surface analysis including mass spectrometric imaging. Lateral resolution in tens of micrometers was demonstrated for desorption nanoelectrospray previously, but sensitivity of the surface scan can be an issue. For desorption electrospray, the drag force in the source is driven by the flow of used gases and vacuum suction. Ion signal intensity can be improved by controlling the nebulizing gas flow rate or auxiliary pumping of a closed compartment in front of the mass spectrometer inlet. Because nanoelectrospray generates charged droplets without the assistance of a nebulizing gas, only vacuum suction drives the gas flow. In this study, the effect of pressure drop between the atmospheric and evacuated region of a mass spectrometer on the ion signal intensity was investigated for desorption nanoelectrospray. A modification of the commercial inlet was designed. An auxiliary pump was directly connected to an inner compartment of the modified mass spectrometer inlet through a needle valve that enabled the regulation of the reduced pressure. Adjustment of the pressure drop significantly increased signal intensity (more than one order of magnitude in some cases). To a lesser extent, the temperature of a heated capillary (an integral part of the inlet) also influenced the signal intensity. The applicability of desorption nanoelectrospray equipped with pressure regulation was demonstrated by the analysis of synthetic cathinones or a pill of paracetamol. Because pressure in the inlet depends on the diameters of orifices and the power of vacuum systems of mass spectrometers, the effect of the pressure regulation can be different for different instruments. Nevertheless, the presented results confirmed the importance of pressure drop-driven transport for desorption nanoelectrospray efficiency and can encourage its new applications.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/EF16_019%2F0000754" target="_blank" >EF16_019/0000754: Nanotechnologies for Future</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
JOURNAL OF MASS SPECTROMETRY
ISSN
1076-5174
e-ISSN
—
Volume of the periodical
55
Issue of the periodical within the volume
10
Country of publishing house
GB - UNITED KINGDOM
Number of pages
7
Pages from-to
"e4642-1"-"e4642-7"
UT code for WoS article
000577099400001
EID of the result in the Scopus database
2-s2.0-85090820782