Comprehensive Characterization of Secondary Metabolites from Colebrookea oppositifolia (Smith) Leaves from Nepal and Assessment of Cytotoxic Effect and Anti-Nf-kappa B and AP-1 Activities In Vitro
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F20%3A73604544" target="_blank" >RIV/61989592:15310/20:73604544 - isvavai.cz</a>
Result on the web
<a href="https://www.mdpi.com/1422-0067/21/14/4897/htm" target="_blank" >https://www.mdpi.com/1422-0067/21/14/4897/htm</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/ijms21144897" target="_blank" >10.3390/ijms21144897</a>
Alternative languages
Result language
angličtina
Original language name
Comprehensive Characterization of Secondary Metabolites from Colebrookea oppositifolia (Smith) Leaves from Nepal and Assessment of Cytotoxic Effect and Anti-Nf-kappa B and AP-1 Activities In Vitro
Original language description
Here we report the comprehensive characterization of the secondary metabolites from the leaves ofColebrookea oppositifoliaSmith, a species used as medicinal plant in the traditional medicine of Nepal. Phytochemical screening of bioactives was performed using an integrated LC-MS(n)and high resolution MS (Mass Spectrometry) approach. Forty-three compounds were tentatively identified, mainly aglyconic and glycosilated flavonoids and phenolic acids, as well as other bioactives such as coumarins and terpenes were detected. Furthermore, the NF-kappa B and AP-1 inhibitory activity ofC. oppositifoliaextract were evaluated, as well as its cytotoxicity against THP-1 cells, in order to assess the potential use of this herb as a source of anti-inflammatory and cytotoxic compounds. The results so far obtained indicate thatC. oppositifolialeaves extract could significantly reduce the viability of THP-1 cells (IC50= 6.2 +/- 1.2 mu g/mL), as well as the activation of both NF-kappa B and AP-1 at the concentration of 2 mu g/mL. Our results indicate that NepaleseC. oppositifoliais a valuable source of anti-inflammatory and cytotoxic compounds. The phytochemical composition reported here can partially justify the traditional uses ofC. oppositifoliain Nepal, especially in the treatment of inflammatory diseases, although further research will be needed to assess the full potential of this species.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30104 - Pharmacology and pharmacy
Result continuities
Project
<a href="/en/project/LO1305" target="_blank" >LO1305: Development of the center of advanced technologies and materials</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
ISSN
1422-0067
e-ISSN
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Volume of the periodical
21
Issue of the periodical within the volume
14
Country of publishing house
CH - SWITZERLAND
Number of pages
15
Pages from-to
"4897-1"-"4897-15"
UT code for WoS article
000556487000001
EID of the result in the Scopus database
2-s2.0-85087741434