Super-resolution imaging of microtubules in Medicago sativa
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F20%3A73604778" target="_blank" >RIV/61989592:15310/20:73604778 - isvavai.cz</a>
Result on the web
<a href="https://obd.upol.cz/id_publ/333184664" target="_blank" >https://obd.upol.cz/id_publ/333184664</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/bs.mcb.2020.03.004" target="_blank" >10.1016/bs.mcb.2020.03.004</a>
Alternative languages
Result language
angličtina
Original language name
Super-resolution imaging of microtubules in Medicago sativa
Original language description
Study of microtubules on cellular and subcellular levels is compromised by limited resolution of conventional fluorescence microscopy. However, it is possible to improve Abbe's diffraction-limited resolution by employment of super-resolution microscopy methods. Two of them, described herein, are structured-illumination microscopy (SIM) and Airyscan laser scanning microscopy (AM). Both methods allow high-resolution imaging of cortical microtubules in plant cells, thus contributing to the current knowledge on plant morphogenesis, growth and development. Both SIM and AM provide certain advantages and characteristic features, which are described here. We present immunofluorescence localization methods for microtubules in fixed plant cells achieving high signal efficiency, superb sample stability and sub-diffraction resolution. These protocols were developed for whole-mount immunolabeling of root samples of legume crop species Medicago sativa. They also contain tips for optimal sample preparation of plants germinated from seeds as well as plantlets regenerated from somatic embryos in vitro. We describe in detail all steps of optimized protocols for sample preparation, microtubule immunolabeling and super-resolution imaging.
Czech name
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Czech description
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Classification
Type
C - Chapter in a specialist book
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
<a href="/en/project/EF16_019%2F0000827" target="_blank" >EF16_019/0000827: Plants as a tool for sustainable global development</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Book/collection name
Methods in cell biology
ISBN
978-0-12-821533-3
Number of pages of the result
15
Pages from-to
237-251
Number of pages of the book
436
Publisher name
Academic Press Inc.
Place of publication
Cambridge, USA
UT code for WoS chapter
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