The impact of graphene oxide on androgen receptor signalling in prostate cancer cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F21%3A73602181" target="_blank" >RIV/61989592:15310/21:73602181 - isvavai.cz</a>
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S004565352032957X" target="_blank" >https://www.sciencedirect.com/science/article/pii/S004565352032957X</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.chemosphere.2020.128759" target="_blank" >10.1016/j.chemosphere.2020.128759</a>
Alternative languages
Result language
angličtina
Original language name
The impact of graphene oxide on androgen receptor signalling in prostate cancer cells
Original language description
Androgen receptor (AR) signalling is triggered by androgens that have lipophilic nature. Since it was indicated that graphene oxide (GO) might facilitate passive diffusion of lipophilic compounds probably via Trojan horse-like mechanism, we tested the hypothesis if this suggestion would apply for androgens as well. Thus, we investigated if GO affects dihydrotestosterone (DHT)-triggered signalling of AR in two prostate cancer-derived cell lines, 22Rv1 and LNCaP. These cell lines differ in number of AR variants, i.e. there are two variants in 22Rv1 cells (full length and truncated) but only one in LNCaP cells (full length). Graphene oxide had no effect on basal luciferase activity but significantly decreased DHT-inducible ARdependent luciferase activity in stably transfected cells. In 22Rv1 cells, it induced concentration-dependent decrease of DHT-inducible KLK3 mRNA and PSA protein after 24 h. While there was no effect on UBE2C mRNA (regulated by truncated variant), there was synergistic effect of DHT and GO on UBE2C protein level. Translocation of full-length AR (AR-FL) was potentiated by GO in the presence of DHT in 22Rv1 cells but it was suppressed in LNCaP cells. DHT-stimulated enrichment of AR-FL on KLK3 promoter was not significantly affected by GO in any tested cell line neither was KLK3 mRNA at 4 h of incubation. In conclusion, GO affects DHT-triggered signalling in both types of cells in similar manner, but ligand-triggered redistribution of AR-FL is affected differently. One of the reasons may be the presence of truncated variant of androgen receptor.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10511 - Environmental sciences (social aspects to be 5.7)
Result continuities
Project
<a href="/en/project/GA19-22720S" target="_blank" >GA19-22720S: Novel Nano-structured Materials for Elimination of Highly- and Multi-resistant Bacteria and for Overcoming Antibiotic Resistance</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
CHEMOSPHERE
ISSN
0045-6535
e-ISSN
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Volume of the periodical
269
Issue of the periodical within the volume
APR
Country of publishing house
GB - UNITED KINGDOM
Number of pages
10
Pages from-to
"128759-1"-"128759-10"
UT code for WoS article
000631725000076
EID of the result in the Scopus database
2-s2.0-85095723865