Tocopherol controls D1 amino acid oxidation by oxygen radicals in Photosystem II.
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F21%3A73607818" target="_blank" >RIV/61989592:15310/21:73607818 - isvavai.cz</a>
Result on the web
<a href="https://www.pnas.org/content/118/4/e2019246118" target="_blank" >https://www.pnas.org/content/118/4/e2019246118</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1073/pnas.2019246118" target="_blank" >10.1073/pnas.2019246118</a>
Alternative languages
Result language
angličtina
Original language name
Tocopherol controls D1 amino acid oxidation by oxygen radicals in Photosystem II.
Original language description
Photosystem II (PSII) is an intrinsic membrane protein complex that functions as a light-driven water:plastoquinone oxidoreductase in oxygenic photosynthesis. Electron transport in PSII is associated with formation of reactive oxygen species (ROS) responsible for oxidative modifications of PSII proteins. In this study, oxidative modifications of the D1 and D2 proteins by the superoxide anion (O-2(center dot-)) and the hydroxyl (HO center dot) radicals were studied in WT and a tocopherol cyclase (vtel) mutant, which is deficient in the lipid-soluble antioxidant alpha-tocopherol. In the absence of this antioxidant, high-resolution tandem mass spectrometry was used to identify oxidation of D1:E-130 to hydroxyglutamic acid by O-2(center dot- ) at the Pheopi site. Additionally, D1:Y-246 was modified to either tyrosine hydroperoxide or dihydroxyphenylalanine by O(2)(center dot- )and HO center dot, respectively, in the vicinity of the nonheme iron. We propose that a-tocopherol is localized near Pheopi and the nonheme iron, with its chromanol head exposed to the lipid-water interface. This helps to prevent oxidative modification of the amino acid's hydrogen that is bonded to Pheopi and the nonheme iron (via bicarbonate), and thus protects electron transport in PSII from ROS damage.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10610 - Biophysics
Result continuities
Project
<a href="/en/project/EF16_019%2F0000827" target="_blank" >EF16_019/0000827: Plants as a tool for sustainable global development</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
ISSN
0027-8424
e-ISSN
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Volume of the periodical
118
Issue of the periodical within the volume
4
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
"2019246118-1"-"2019246118-10"
UT code for WoS article
000612945500056
EID of the result in the Scopus database
2-s2.0-85100011586