In vivo light-sheet microscopy resolves localisation patterns of FSD1, a superoxide dismutase with function in root development and osmoprotection
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F21%3A73607845" target="_blank" >RIV/61989592:15310/21:73607845 - isvavai.cz</a>
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/10.1111/pce.13894" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1111/pce.13894</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/pce.13894" target="_blank" >10.1111/pce.13894</a>
Alternative languages
Result language
angličtina
Original language name
In vivo light-sheet microscopy resolves localisation patterns of FSD1, a superoxide dismutase with function in root development and osmoprotection
Original language description
Superoxide dismutases (SODs) are enzymes detoxifying superoxide to hydrogen peroxide while temporal developmental expression and subcellular localisation are linked to their functions. Therefore, we aimed here to revealin vivodevelopmental expression, subcellular, tissue- and organ-specific localisation of iron superoxide dismutase 1 (FSD1) inArabidopsisusing light-sheet and Airyscan confocal microscopy. FSD1-GFP temporarily accumulated at the site of endosperm rupture during seed germination. In emerged roots, it showed the highest abundance in cells of the lateral root cap, columella, and endodermis/cortex initials. The largest subcellular pool of FSD1-GFP was localised in the plastid stroma, while it was also located in the nuclei and cytosol. The majority of the nuclear FSD1-GFP is immobile as revealed by fluorescence recovery after photobleaching. We found thatfsd1knockout mutants exhibit reduced lateral root number and this phenotype was reverted by genetic complementation. Mutant analysis also revealed a requirement for FSD1 in seed germination during salt stress. Salt stress tolerance was coupled with the accumulation of FSD1-GFP in Hechtian strands and superoxide removal. It is likely that the plastidic pool is required for acquiring oxidative stress tolerance inArabidopsis. This study suggests new developmental and osmoprotective functions of SODs in plants.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
PLANT CELL AND ENVIRONMENT
ISSN
0140-7791
e-ISSN
—
Volume of the periodical
44
Issue of the periodical within the volume
1
Country of publishing house
GB - UNITED KINGDOM
Number of pages
20
Pages from-to
68-87
UT code for WoS article
000577432300001
EID of the result in the Scopus database
2-s2.0-85092197649