Scanning electrochemical microscopy-somatic cell count as a method for diagnosis of bovine mastitis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F22%3A73614818" target="_blank" >RIV/61989592:15310/22:73614818 - isvavai.cz</a>
Result on the web
<a href="https://www.mdpi.com/2079-7737/11/4/549/htm" target="_blank" >https://www.mdpi.com/2079-7737/11/4/549/htm</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/biology11040549" target="_blank" >10.3390/biology11040549</a>
Alternative languages
Result language
angličtina
Original language name
Scanning electrochemical microscopy-somatic cell count as a method for diagnosis of bovine mastitis
Original language description
The method to diagnose mastitis is generally the somatic cell count (SCC) by flow cytometry measurement. When the number of somatic cells in raw milk is 2.0 × 105 cells/mL or more, the condition is referred to as mastitis. In the current study, we created a milk cell chip that serves as an electrochemical method that can be easily produced and used utilizing scanning electrochemical microscopy (SECM). The microelectrode present in the cell chip scans, and the difference between the oxygen concentration near the milk cell chip and in bulk is measured as the oxygen (O2) reduction current. We estimated the relationship between respiratory activity and the number of somatic cells in raw milk as a calibration curve, using scanning electrochemical microscopy-somatic cell count (SECM-SCC). As a result, a clear correlation was shown in the range of 104 cells/mL to 106 cells/mL. The respiration rate (F) was estimated to be about 10–16 mol/s per somatic cell. We also followed the increase in oxygen consumption during the respiratory burst using differentiation inducer phorbol 12-myristate 13-acetate (PMA) as an early stage of mastitis, accompanied with an increase in immune cells, which showed similar results. In addition, we were able to discriminate between cattle with mastitis and without mastitis.
Czech name
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Czech description
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Classification
Type
J<sub>SC</sub> - Article in a specialist periodical, which is included in the SCOPUS database
CEP classification
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OECD FORD branch
10601 - Cell biology
Result continuities
Project
<a href="/en/project/EF16_019%2F0000827" target="_blank" >EF16_019/0000827: Plants as a tool for sustainable global development</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biology
ISSN
2079-7737
e-ISSN
2079-7737
Volume of the periodical
11
Issue of the periodical within the volume
4
Country of publishing house
CH - SWITZERLAND
Number of pages
15
Pages from-to
"549-1"-"549-15"
UT code for WoS article
000786218800001
EID of the result in the Scopus database
2-s2.0-85128421986