Cooling storage of ram sperm in presence of antioxidant glutathione.
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F22%3A73616581" target="_blank" >RIV/61989592:15310/22:73616581 - isvavai.cz</a>
Result on the web
<a href="https://cjas.agriculturejournals.cz/pdfs/cjs/2022/09/02.pdf" target="_blank" >https://cjas.agriculturejournals.cz/pdfs/cjs/2022/09/02.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.17221/113/2022-CJAS" target="_blank" >10.17221/113/2022-CJAS</a>
Alternative languages
Result language
angličtina
Original language name
Cooling storage of ram sperm in presence of antioxidant glutathione.
Original language description
In the process of cryopreservation, the semen of animals can be threatened by many factors, such as oxidative damage, which affect the motility and plasma membrane function of the sperm. As an endogenous antioxidant in animals, glutathione (GSH) can significantly improve the quality of thawed sperm when added to the diluent of the semen. This study was aimed at examining the effect of GSH (given at 0.5 mM, 1.5 mM and 5 mM) on the motility and viability of ram sperm following storage under cooling conditions for several days. GSH reduced the negative effect of the cooling conditions on the total and progressive motility (CASA), stability of the membranes (annexin V/PI) and incidence of the apoptosis/necrosis (Yo-Pro-1/PI) in the sperm maintained at 5 °C for 24 h and 48 hours. These results indicate that the addition of GSH to the sperm extender can maintain the quality of the ram sperm stored at cooling conditions for at least two days.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10604 - Reproductive biology (medical aspects to be 3)
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
CZECH JOURNAL OF ANIMAL SCIENCE
ISSN
1212-1819
e-ISSN
1805-9309
Volume of the periodical
67
Issue of the periodical within the volume
9
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
9
Pages from-to
"356 "- 364
UT code for WoS article
000868999900002
EID of the result in the Scopus database
2-s2.0-85142800096