Molecular basis of differential adventitious rooting competence in poplar genotypes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F22%3A73616735" target="_blank" >RIV/61989592:15310/22:73616735 - isvavai.cz</a>

Result on the web

<a href="https://academic.oup.com/jxb/article/73/12/4046/6553347" target="_blank" >https://academic.oup.com/jxb/article/73/12/4046/6553347</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/jxb/erac126" target="_blank" >10.1093/jxb/erac126</a>

Result language

angličtina

Original language name

Molecular basis of differential adventitious rooting competence in poplar genotypes

Original language description

Recalcitrant adventitious root (AR) development is a major hurdle in propagating commercially important woody plants. Although significant progress has been made to identify genes involved in subsequent steps of AR development, the molecular basis of differences in apparent recalcitrance to form AR between easy-to-root and difficult-to-root genotypes remains unknown. To address this, we generated cambium tissue-specific transcriptomic data from stem cuttings of hybrid aspen, T89 (difficult-to-root) and hybrid poplar OP42 (easy-to-root), and used transgenic approaches to verify the role of several transcription factors in the control of adventitious rooting. Increased peroxidase activity was positively correlated with better rooting. We found differentially expressed genes encoding reactive oxygen species scavenging proteins to be enriched in OP42 compared with T89. A greater number of differentially expressed transcription factors in cambium cells of OP42 compared with T89 was revealed by a more intense transcriptional reprograming in the former. PtMYC2, a potential negative regulator, was less expressed in OP42 compared with T89. Using transgenic approaches, we demonstrated that PttARF17.1 and PttMYC2.1 negatively regulate adventitious rooting. Our results provide insights into the molecular basis of genotypic differences in AR and implicate differential expression of the master regulator MYC2 as a critical player in this process.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10611 - Plant sciences, botany

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

JOURNAL OF EXPERIMENTAL BOTANY

ISSN

0022-0957

e-ISSN

1460-2431

Volume of the periodical

73

Issue of the periodical within the volume

12

Country of publishing house

GB - UNITED KINGDOM

Number of pages

19

Pages from-to

4046-4064

UT code for WoS article

000813477900001

EID of the result in the Scopus database

2-s2.0-85133797842