Effect of ion fluxes on regulating the light-induced transthylakoid electric potential difference.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F23%3A73614824" target="_blank" >RIV/61989592:15310/23:73614824 - isvavai.cz</a>

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S0981942822004880" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0981942822004880</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.plaphy.2022.10.028" target="_blank" >10.1016/j.plaphy.2022.10.028</a>

Result language

angličtina

Original language name

Effect of ion fluxes on regulating the light-induced transthylakoid electric potential difference.

Original language description

The light-induced transthylakoid membrane potential (ΔΨ) can not only drive the ATP synthesis through the ATP-synthase in chloroplasts but serve as an essential modifier in the acclimation of photosynthesis to fluctuating light conditions. It has been manifested that during photosynthesis, the light-induced ΔΨ is responsive to multiple factors among which the ion channels/transporters (e.g., V–K+, VCCN1, and KEA3) are key to adjust the ion distribution on the two sides of the thylakoid membrane and hence shape the kinetics of ΔΨ. However, an in-depth mechanistic understanding of ion fluxes on adjusting the transthylakoid electric potentials is still unclear. This lack of a mechanistic understanding is due to the experimental difficulty of closely observing ion fluxes in vivo and also hacking the evolution of parameters in a highly intertwined photosynthetic network. In this work, a computer model was applied to investigate the roles of ion fluxes on adjusting transthylakoid electric potentials upon a temporal cycle of a period of high illumination followed by a dark-adapted phase. The computing data revealed that, firstly, upon illumination, the dissipation of the steady-ΔΨ by ∼10 mV is contributed from the V–K+-driven K+ flux whilst ∼8 mV of the steady-ΔΨ is dissipated by the VCCN1-pumped Cl− flux, but there were no appreciable KEA3-evoked variations on ΔΨ; secondly, on transition from high light to darkness, V–K+ and KEA3 are serving as major contributors whereas VCCN1 taking a counterbalancing part in shaping a standard trace of ECS (electrochromic shift), which commonly shows a sharp fall to a minimum before returning to the baseline in darkness. Besides, the functional consequences on components of ΔΨ adjusted by every particular ion channel/transporter were also explored. By employing the model, we bring evidence that particular thylakoid-harbored proteins, namely V–K+, VCCN1, and KEA3, function by distinct mechanisms in the dynamic adjustment of electric potential, which might be mainly importnat under fluctuating light conditions.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10610 - Biophysics

Project

<a href="/en/project/EF16_019%2F0000827" target="_blank" >EF16_019/0000827: Plants as a tool for sustainable global development</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

PLANT PHYSIOLOGY AND BIOCHEMISTRY

ISSN

0981-9428

e-ISSN

1873-2690

Volume of the periodical

194

Issue of the periodical within the volume

JAN

Country of publishing house

FR - FRANCE

Number of pages

10

Pages from-to

60-69

UT code for WoS article

000895736600006

EID of the result in the Scopus database

2-s2.0-85141750809