Spatiotemporal distribution of reactive oxygen species production, delivery, and use in Arabidopsis root hairs
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F23%3A73621566" target="_blank" >RIV/61989592:15310/23:73621566 - isvavai.cz</a>
Result on the web
<a href="https://academic.oup.com/plphys/article-pdf/193/4/2337/53684090/kiad484.pdf" target="_blank" >https://academic.oup.com/plphys/article-pdf/193/4/2337/53684090/kiad484.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/plphys/kiad484" target="_blank" >10.1093/plphys/kiad484</a>
Alternative languages
Result language
angličtina
Original language name
Spatiotemporal distribution of reactive oxygen species production, delivery, and use in Arabidopsis root hairs
Original language description
Fluorescent selective probes for reactive oxygen species (ROS) detection in living cells are versatile tools for the documentation of ROS production in plant developmental or stress reactions. We employed high-resolution live-cell imaging and semiquantitative analysis of Arabidopsis (Arabidopsis thaliana) stained with CM-H2DCFDA, CellROX Deep Red, and Amplex Red for functional characterization of the spatiotemporal mode of ROS production, delivery, and utilization during root hair formation. Cell viability marker fluorescein diacetate served as a positive control for dye loading and undisturbed root hair tip growth after staining. Using a colocalization analysis with subcellular molecular markers and two root hair mutants with similar phenotypes of nonelongating root hairs, but with contrasting reasons for this impairment, we found that: (i) CM-H2DCFDA is a sensitive probe for ROS generation in the cytoplasm, (ii) CellROX Deep Red labels ROS in mitochondria, (iii) Amplex Red labels apoplastic ROS and mitochondria and shows high selectivity to root hairs, (iv) the root hair defective 2-1 (rhd2-1) mutant with nonfunctional NADPH oxidase RESPIRATORY BURST OXIDASE HOMOLOG PROTEIN C/ROOT HAIR-DEFECTIVE 2 (AtRBOHC/RHD2) has a low level of CM-H2DCFDA-reactive ROS in cytoplasm and lacks Amplex Red-reactive ROS in apoplast, and (v) the ACTIN2-deficient deformed root hairs1-3 (der1-3) mutant is not altered in these aspects. The sensitivity of CellROX Deep Red was documented by discrimination between larger ROS-containing mitochondria and small, yet ROS-free premature mitochondria in the growing tip of root hairs. We characterized spatial changes in ROS production and compartmentalization induced by external ROS modulators, ethylene precursor 1-aminocyclopropane-1-carboxylic acid, and ionophore valinomycin. This dynamic and high-resolution study of ROS production and utilization opens opportunities for precise speciation of particular ROS involved in root hair formation.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
<a href="/en/project/GA19-18675S" target="_blank" >GA19-18675S: Clarification of actin, NADPH oxidase and structural sterol roles in root hair tip growth using advanced microscopy and proteomics</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
PLANT PHYSIOLOGY
ISSN
0032-0889
e-ISSN
1532-2548
Volume of the periodical
193
Issue of the periodical within the volume
4
Country of publishing house
US - UNITED STATES
Number of pages
24
Pages from-to
2337-2360
UT code for WoS article
001066321000001
EID of the result in the Scopus database
2-s2.0-85178544778