Chiral sensing combined with nuclease activity assay to track Cas9 dynamics in solution: ROA and CPL study

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F25%3A73630938" target="_blank" >RIV/61989592:15310/25:73630938 - isvavai.cz</a>

Result on the web

<a href="https://pubs.rsc.org/en/content/articlelanding/2025/cc/d5cc00971e" target="_blank" >https://pubs.rsc.org/en/content/articlelanding/2025/cc/d5cc00971e</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/d5cc00971e" target="_blank" >10.1039/d5cc00971e</a>

Result language

angličtina

Original language name

Chiral sensing combined with nuclease activity assay to track Cas9 dynamics in solution: ROA and CPL study

Original language description

Chiroptical studies of the SpyCas9 protein are extremely rare. Nondestructive methods are needed to characterize its active ribonucleoprotein form. Using Raman optical activity (ROA) and circularly polarized luminescence (CPL), we present a new approach to detect key biomolecules involved in CRISPR-Cas technology while preserving their original nucleolytic activity.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10306 - Optics (including laser optics and quantum optics)

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

CHEMICAL COMMUNICATIONS

ISSN

1359-7345

e-ISSN

1364-548X

Volume of the periodical

61

Issue of the periodical within the volume

38

Country of publishing house

GB - UNITED KINGDOM

Number of pages

4

Pages from-to

6905-6908

UT code for WoS article

001466652700001

EID of the result in the Scopus database

2-s2.0-105003077693