Binding of ligands to the aryl hydrocarbon receptor: An overview of methods
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F25%3A73631533" target="_blank" >RIV/61989592:15310/25:73631533 - isvavai.cz</a>
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0378427425000098" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0378427425000098</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.toxlet.2025.01.003" target="_blank" >10.1016/j.toxlet.2025.01.003</a>
Alternative languages
Result language
angličtina
Original language name
Binding of ligands to the aryl hydrocarbon receptor: An overview of methods
Original language description
The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor, which plays numerous and pivotal roles in human physiology and pathophysiology. Therefore, pharmacotherapeutic targeting of the AhR is a highly pertinent issue. The identification of new AhR ligands and the characterization of the interactions between the AhR ligands and AhR protein requires appropriate methodology. In spite the AhR is monomeric intracellular soluble receptor, the full-length human AhR protein has not been crystallized so far, and its isolation in a form applicable in the binding assays is highly challenging. Recent advances, including crystallization of AhR fragments, recombinant protein technologies, and cryogenic electron microscopy, allowed for exploitation of diverse experimental techniques for studying interactions between ligands and the AhR. In the current paper, we review existing AhR ligand binding assays, including their description, applicability and limitations.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30108 - Toxicology
Result continuities
Project
<a href="/en/project/GA23-04662S" target="_blank" >GA23-04662S: Dietary monoterpenoids as a novel class of aryl hydrocarbon receptor negative allosteric modulators in the therapy of colon cancer</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
TOXICOLOGY LETTERS
ISSN
0378-4274
e-ISSN
1879-3169
Volume of the periodical
404
Issue of the periodical within the volume
FEB
Country of publishing house
IE - IRELAND
Number of pages
10
Pages from-to
37-46
UT code for WoS article
001407494600001
EID of the result in the Scopus database
2-s2.0-85215417650