On the limits of applicability of spectrophotometric and spectrofluorimetric methods for the determination of chlorophyll a/b ratio

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F99%3A00000803" target="_blank" >RIV/61989592:15310/99:00000803 - isvavai.cz</a>

Alternative codes found

RIV/60076658:12640/99:00004752

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

On the limits of applicability of spectrophotometric and spectrofluorimetric methods for the determination of chlorophyll a/b ratio

Original language description

The concentration limits for spectrophotometric and spectrofluorimetric determinations of the chlorophyll (Chl) a/b ratio in barley leaves were studied using 80% acetone extracts at room temperature. The optimum sample absorbances (at 663.2 nm - maximumof the QY band of Chl a) for the Chl a/b determination were determined. For given spectrometers and sample positions these absorbances ranged between 0.2 and 1.0 and 0.008 - 0.1 for the absorption and fluorescence methods, respectively. Precision of themeasurements and the distorting effects are discussed. The lower limits of both absorption and fluorescence methods depend on sensitivity of the spectrometers for the Chl b detection. The spectrophotometric determination of Chl a/b ratio at higher Chl c oncentrations can be distorted by the chlorophyll fluorescence signal. The extent of this distortion depends on sample-detector geometry in any given type of the spectrometer. The effect of inner filter of Chl molecules and the detection i

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

BO - Biophysics

OECD FORD branch

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Project

<a href="/en/project/VS96085" target="_blank" >VS96085: Studies of relations between function of biological membranes and interactions of their components</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

1999

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Photosynthesis Research

ISSN

0166-8595

e-ISSN

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Volume of the periodical

62

Issue of the periodical within the volume

1

Country of publishing house

XX - stateless person

Number of pages

13

Pages from-to

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UT code for WoS article

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EID of the result in the Scopus database

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