Pea seedling aminoaldehyde dehydrogenase: primary structure and active site residues

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15410%2F03%3A00001326" target="_blank" >RIV/61989592:15410/03:00001326 - isvavai.cz</a>

Alternative codes found

RIV/61989592:15310/03:00001326

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Pea seedling aminoaldehyde dehydrogenase: primary structure and active site residues

Original language description

The first primary structure of a plant aminoaldehyde dehydrogenase (AMADH, EC 1.2.1.19) is reported. The enzyme of pea (Pisum sativum) seedlings subjected to our study oxidises omega-aminoaldehydes to the corresponding omega-amino acids. Although pea does not accumulate betaine aldehyde as a compatible osmolyte, the N-terminal sequence of a purified pea AMADH resembles those of plant betaine aldehyde dehydrogenases (BADHs). On the basis of an anticipated pea AMADH homology to these enzymes, degeneratedoligonucleotide primers were designed and used for PCR amplification. Two cDNA fragments were obtained in initial 5'RACE experiments. Subsequent 5' and 3' RACE performed with specific non-degenerated primers provided two putative cDNAs of the plant BADHfamily. Both encoded protein sequences (AMADH I and AMADH2) are highly homologous to those of plant BADHs. They show 81% identity and 92% in mutual alignment. As a deduced product of the first cDNA, AMADH1 completely matches the N-termina

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

<a href="/en/project/GA203%2F99%2FD048" target="_blank" >GA203/99/D048: Purification of plant aminoaldehyde dehydrogenase and its enzymological characterization</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2003

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Plant Physiology and Biochemistry

ISSN

0981-9428

e-ISSN

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Volume of the periodical

41

Issue of the periodical within the volume

1

Country of publishing house

FR - FRANCE

Number of pages

10

Pages from-to

1-10

UT code for WoS article

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EID of the result in the Scopus database

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