Mastering POX genotype for fatty acid transport and accumulation in the yeast Yarrowia lipolytica

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15410%2F03%3A00001452" target="_blank" >RIV/61989592:15410/03:00001452 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Mastering POX genotype for fatty acid transport and accumulation in the yeast Yarrowia lipolytica
Original language description
The yeast Yarrowia lipolytica is able to use fatty acids as carbon source via -oxidation. This yeast contains five acyl-CoA oxidases (Aoxps) encoded by POX genes. Two Aoxps are of particular interest: Aox2p, a medium chain oxidase, and Aox3p, anoxidase specific for short chain substrates. To date, no 3D structure of these enzymes is available. To better understand their specificity, we have expressed 10 enzymes in Escherichia coli; Aox2p, Aox3p, a mutated Aox3 (Aox3mp), and seven chimera obtained by shuffling fragments of Aox2p and Aox3p (M1 to M4 and M6 to M8). Aox3mp and one chimeric protein (M6) were active, Aox3mp exhibited broad chain length specificity. We tested complementation of growth for strain MTLY36 (..pox2,3,5; reduced growth onoleic acid media) and MTLY40 (..pox2,3,4,5; not growing on oleic acid media) by expressing either Aox2p, Aox3p, Aox3mp, and M6 chimera encoding genes. We demonstrate that the POX genomic content affects both -lactone production [J
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Publication year
2003
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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