Time- and dose-dependent seeding tendency of exogenous tau R2 and R3 aggregates in cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15640%2F23%3A73618356" target="_blank" >RIV/61989592:15640/23:73618356 - isvavai.cz</a>

Alternative codes found

RIV/61989592:15110/23:73618356

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S0006291X23002425?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0006291X23002425?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.bbrc.2023.02.057" target="_blank" >10.1016/j.bbrc.2023.02.057</a>

Result language

angličtina

Original language name

Time- and dose-dependent seeding tendency of exogenous tau R2 and R3 aggregates in cells

Original language description

Tauopathies are a group of neurodegenerative diseases categorised into three types, 3R, 4R, or 3Rþ4R (mixed) tauopathies, based on the tau isoforms that make up the aberrant filaments. It is supposed that all six tau isoforms share functional characteristics. However, differences in the neuropathological features associated with different tauopathies offer the possibility that disease progression and tau accumulation may vary depending on the isoform composition. The presence or absence of repeat 2 (R2) in the microtubule-binding domain defines the type of isoform, which might influence tau pathology associated with a particular tau isoform. Therefore, our study aimed to identify the differences in the seeding propensities of R2 and repeat 3 (R3) aggregates using HEK293T biosensor cells. We show that the seeding induced by R2 was generally higher than by R3 aggregates, and lower concentrations of R2 aggregates are sufficient to induce seeding. Next, we found that both R2 and R3 aggregates dosedependently increased triton-insoluble Ser262 phosphorylation of native tau, which is only visible in cells seeded with higher concentrations (12.5 nM or 100 nM) of R2 and R3 aggregates, despite the seeding by the lower concentrations of R2 aggregates after 72 h. However, the accumulation of tritoninsoluble pSer262 tau was visible earlier in cells induced with R2 than in R3 aggregates. Our findings suggest that the R2 region may contribute to the early and enhanced induction of tau aggregation and define the difference in disease progression and neuropathology of 4R tauopathies.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

ISSN

0006-291X

e-ISSN

1090-2104

Volume of the periodical

653

Issue of the periodical within the volume

31.03.2023

Country of publishing house

US - UNITED STATES

Number of pages

4

Pages from-to

102-105

UT code for WoS article

000949585300001

EID of the result in the Scopus database

2-s2.0-85150430796