Mechanistic insights into interactions between ionizable lipid nanodroplets and biomembranes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15640%2F25%3A73625003" target="_blank" >RIV/61989592:15640/25:73625003 - isvavai.cz</a>
Alternative codes found
RIV/61989100:27740/25:10254766
Result on the web
<a href="https://www.tandfonline.com/doi/full/10.1080/07391102.2024.2329307" target="_blank" >https://www.tandfonline.com/doi/full/10.1080/07391102.2024.2329307</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1080/07391102.2024.2329307" target="_blank" >10.1080/07391102.2024.2329307</a>
Alternative languages
Result language
angličtina
Original language name
Mechanistic insights into interactions between ionizable lipid nanodroplets and biomembranes
Original language description
Delivery of RNA into cells using lipid nanoparticles (LNPs) has been a significant breakthrough in RNA-based medicine, with clinical applicability expanded through the use of ionizable lipids (ILs). These unique lipids can alter their charge state in response to pH changes, which is crucial for pH-triggered endosomal escape and effective lipid-mediated RNA delivery. In this study, we conducted a comprehensive set of molecular dynamics (MD) simulations to investigate interactions between IL-containing lipid nanodroplets (LNDs) and cell membrane models. Using an atomistic resolution model, we investigated the merging process of LNDs with cell membrane models under neutral conditions relevant to an intercellular environment and acidic pH conditions found in late endosomes. Our observations revealed that at neutral pH, LNDs merged with lipid membranes while preserving the bilayer structure. Under acidic conditions, the LNDs remained attached to the bilayer without fusing into the membranes. Importantly, the presence of ILs did not disrupt the original biomembrane structure during the simulation period. The MD simulations provided valuable atomistic insights into the mechanism of interaction between IL-containing nanodroplets and biomembranes, which could aid the rational design of ILs to develop more efficient LNPs for RNA therapies.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10403 - Physical chemistry
Result continuities
Project
<a href="/en/project/EH22_008%2F0004587" target="_blank" >EH22_008/0004587: Technology Beyond Nanoscale</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
ISSN
0739-1102
e-ISSN
1538-0254
Volume of the periodical
43
Issue of the periodical within the volume
17
Country of publishing house
GB - UNITED KINGDOM
Number of pages
11
Pages from-to
nestránkováno
UT code for WoS article
001185421500001
EID of the result in the Scopus database
2-s2.0-85188468956