A Rapid Approach for Identifying Cell Lines Lacking Functional Cytidine Deaminase
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15640%2F25%3A73630558" target="_blank" >RIV/61989592:15640/25:73630558 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15110/25:73630558
Result on the web
<a href="https://www.mdpi.com/1422-0067/26/7/3344" target="_blank" >https://www.mdpi.com/1422-0067/26/7/3344</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/ijms26073344" target="_blank" >10.3390/ijms26073344</a>
Alternative languages
Result language
angličtina
Original language name
A Rapid Approach for Identifying Cell Lines Lacking Functional Cytidine Deaminase
Original language description
CDD plays a pivotal role within the pyrimidine salvage pathway. In this study, a novel, rapid method for the identification of cell lines lacking functional cytidine deaminase was developed. This innovative method utilizes immunocytochemical detection of the product of 5-fluorocytidine deamination, 5-fluorouridine in cellular RNA, enabling the identification of these cells within two hours. The approach employs an anti-bromodeoxyuridine antibody that also specifically binds to 5-fluorouridine and its subsequent detection by a fluorescently labeled antibody. Our results also revealed a strong correlation between the 5-fluorouridine/5-fluorocytidine cytotoxicity ratio and cytidine deaminase content. On the other hand, no correlation was observed between the 5-fluorouridine/5-fluorocytidine cytotoxicity ratio and deoxycytidine monophosphate deaminase content. Similarly, no correlation was observed between this ratio and equilibrative nucleoside transporters 1 or 2. Finally, concentrative nucleoside transporters 1, 2, or 3 also do not correlate with the 5-fluorouridine/5-fluorocytidine cytotoxicity ratio.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
ISSN
1422-0067
e-ISSN
1422-0067
Volume of the periodical
26
Issue of the periodical within the volume
7
Country of publishing house
CH - SWITZERLAND
Number of pages
10
Pages from-to
3344
UT code for WoS article
001463844200001
EID of the result in the Scopus database
2-s2.0-105002286764