Selenium nanoparticles: influence of reducing agents on particle stability and antibacterial activity at biogenic concentrations

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15640%2F25%3A73633212" target="_blank" >RIV/61989592:15640/25:73633212 - isvavai.cz</a>

Alternative codes found

RIV/61989592:15110/25:73633212 RIV/61989592:15310/25:73633212

Result on the web

<a href="https://pubs.rsc.org/en/content/articlepdf/2025/nr/d4nr05271d" target="_blank" >https://pubs.rsc.org/en/content/articlepdf/2025/nr/d4nr05271d</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/d4nr05271d" target="_blank" >10.1039/d4nr05271d</a>

Result language

angličtina

Original language name

Selenium nanoparticles: influence of reducing agents on particle stability and antibacterial activity at biogenic concentrations

Original language description

Selenium nanoparticles (SeNPs) have recently attracted attention for their antimicrobial and anticancer activities. Nevertheless, their use remains limited due to stability issues. The objective of this study is to investigate the impact of different reaction conditions (including the reducing and stabilizing agents, as well as reaction temperature) on the water dispersion characteristics, stability, and biological activity of SeNPs. The particle characteristics were controlled using sodium borohydride as a strong reducing agent and ascorbic acid as a mild agent. The impact of different stabilizers, namely sodium oleate, quercetin, gelatine, poly(ethyleneimine), and poly(diallyldimethyl-ammonium chloride), was investigated on both particle stability and biological activity. Several destabilizing processes occurred, one of which was continuous reduction to the final Se(-II) oxidation state, which was observed in both synthetic approaches, with using sodium borohydride or ascorbic acid as reducing agents. Non-stabilized SeNP dispersions were stable for a maximum of two weeks, while most stabilized SeNP dispersions remained stable for at least two months, and some remained stable for as long as six months. The antibacterial activity had strong effects, particularly against Gram-positive bacteria, and simultaneously low cytotoxicity against mammalian cells. SeNPs exhibited significant antibacterial efficacy, particularly against Staphylococcus aureus, including methicillin-resistant Staphylococcus aureus strains, even at concentrations as low as 1 mg L-1. SeNPs synthesized utilizing sodium borohydride demonstrate minimal cytotoxicity (EC50 &gt; 100 mg L-1). Interestingly, SeNPs reduced by ascorbic acid demonstrated higher cytotoxicity (EC50 6.8 mg L-1) against the NIH/3T3 cell line. This effect is likely due to the combined cytotoxic effect of SeNPs and ascorbic acid acting as a pro-oxidant at high concentrations.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10403 - Physical chemistry

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nanoscale

ISSN

2040-3364

e-ISSN

2040-3372

Volume of the periodical

17

Issue of the periodical within the volume

13

Country of publishing house

GB - UNITED KINGDOM

Number of pages

13

Pages from-to

8170-8182

UT code for WoS article

001440460900001

EID of the result in the Scopus database

2-s2.0-105001689939