Application of computer imaging, stripping voltammetry and mass spectrometry for study of the effect of lead (Pb-EDTA) on growth and viability of early somatic embryos of Norway spruce (Picea abies /L./ Karst.)

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F05%3A00056375" target="_blank" >RIV/62156489:43210/05:00056375 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Application of computer imaging, stripping voltammetry and mass spectrometry for study of the effect of lead (Pb-EDTA) on growth and viability of early somatic embryos of Norway spruce (Picea abies /L./ Karst.)

Original language description

Image analysis (IA) was used to determine the areas and circumferences of clusters of early somatic embryos (ESEs) of the Norway spruce (Picea abies /L./Karst.). Results obtained from IA were compared with the fresh weights of the ESE clusters and theiresterase activities. The areas of the ESE clusters correlated well with both the increases in fresh weight (R2=0.99) of the ESEs and their esterase activities (R2=0.99). In addition, we studied the viability of the ESEs, which was determined by (a) double staining with fluorescein diacetate and propidium iodide (the resulting fluorescence was quantified by IA) and (b) determining esterase activity using a spectrofluorimetric detector. The results obtained with IA and esterase assay were comparable (thedeviation between the tangents of the bisectors was 6.4%). IA was also used to study the effect of Pb--EDTA

Czech name

Využití polčítačového zpracovnán obrazu, rozpouštěcí voltametrie a hmotnostní spektroskopie při studiu vlivu olova na růst a životaschopnost raných soamtických embryí smrku ztepilého (Picea abies /L./ Karst.)

Czech description

Bylo zjištěno, že analýza obrazu je vhodnou metodou pro sledování růstu kultur raných somatických embryí smrku ztepilého (Picea abies /L./ Karst.). Obdobně je možné využít analýzu obrazu při sledování živatoschopnosti s využitím fluorescenčních barviv. Získané metody byly využity při sledování vlivu Pb-EDTA na raná soamtická embrya.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EI - Biotechnology and bionics

OECD FORD branch

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Project

<a href="/en/project/GP525%2F04%2FP132" target="_blank" >GP525/04/P132: Study of protection mechanisms for stress-induced by heavy metals</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Analytical and bioanalytical chemistry

ISSN

1618-2642

e-ISSN

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Volume of the periodical

383

Issue of the periodical within the volume

4

Country of publishing house

DE - GERMANY

Number of pages

11

Pages from-to

576-586

UT code for WoS article

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EID of the result in the Scopus database

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