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Increase in content of metallothionein as marker of resistance to cisplatin treatment

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F06%3A00103447" target="_blank" >RIV/62156489:43210/06:00103447 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Increase in content of metallothionein as marker of resistance to cisplatin treatment

  • Original language description

    Four classes of metallothioneins (MT1, MT2, MT3 and MT4) have been described until now. Human metallothioneins belongs to I. class of metallothioneins (MT1) and are encoded by gene family with 10 isoforms. Due to their high affinity to heavy metals e.g.zinc, copper and/or cadmium, homeostatic control and detoxification of the metals are their main physiological function at evolutionary different animal organisms. Moreover, it has been discussed that expression of MT could relate with resistance to a treatment by anticancer drugs based on heavy metals, most of all, platinum group metals. The aim of this work was to investigate interactions of MT with cisplatin and to determine a content of MT at tumour cell lines. We used cell lines obtained from neuroblastome that were cisplatin sensitive (UKF-NB 2, UKF-NB 3 and UKF-NB 4) and/or cisplatin resistant (UKF-NB 2 CDDP, UKF-NB 3 CDDP and UKF-NB 4 CDDP). The samples were analysed with AUTOLAB Analyser (EcoChemie, Netherlands). The supporting

  • Czech name

    Význam zvýšení obsahu metalothioneinu v rezistenci k léčbě cisplatinou

  • Czech description

    Byli popsány 4 třídy metalothioneinu. Lidský metalothionein patří do první skupiny a jsou kódovány rodinou s deseti isofomami. Pro jejich schopnosti vytvářet komplexy s kovy jako je zinek, kadmium apod. má důležitou fyziologickou funkci v regulaci hladintěchto iontu v organizmu. Je pravděpodobné, že sehrávají úlohu v nádorové rezistenci při léčbě platinovými cytostatiky. V této práci jsme zkoumali interakci platinových cytostatik s MT a koncentraci MT u nádorových liniích. My jsme použily buněčné liniez neuroblastomu a to linie rezistentní (UKF-NB 2 CDDP, UKF-NB 3 CDDP and UKF-NB 4 CDDP) a senzitivní (UKF-NB 2, UKF-NB 3 and UKF-NB 4). Přítomnost cisplatiny u buněčných linií spustí tvorbu thiolových látek, jejich množství vzrostlo až o 1000% v porovnání s kontrolou..

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CG - Electrochemistry

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/GP525%2F04%2FP132" target="_blank" >GP525/04/P132: Study of protection mechanisms for stress-induced by heavy metals</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2006

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Clinical chemistry

  • ISSN

    0009-9147

  • e-ISSN

  • Volume of the periodical

    52

  • Issue of the periodical within the volume

    6

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    2

  • Pages from-to

    "A174"-"A175"

  • UT code for WoS article

  • EID of the result in the Scopus database