Improved method for directed enzyme evolution
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F07%3A00117762" target="_blank" >RIV/62156489:43210/07:00117762 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Improved method for directed enzyme evolution
Original language description
In plants, beta-glucosidases have been implicated in regulating several aspects of development, including phytohormone activation, degradation of endosperm cell wall during germination, and pathogen defense. beta-glucosidases are a widespread group of enzymes that hydrolyze a broad variety of glucosides including aryl- and alkyl-beta-D-glucosides. The maize beta-glucosidase Zm-p60.1 is important for the regulation of plant development through its role in the targeted release of free cytokinins from cytokinin-O-glucosides, their inactive storage forms. For research focused on beta-glucosidase Zm-p60.1 substrate specificity we needed a procedure proving structure stability of the enzyme during mutagenesis. We developed a directed mutagenesis assay usinga system comprising specially designed multi degenerated primers and direct screening of colonies containing positive mutations. This method performed in cycles is a suitable tool for directed evolution of enzymes concretely beta-glucosid
Czech name
Vylepšená metoda pro řízenou evoluci enzymů
Czech description
Byla vyvinuta nová metoda pro řízenou evoluci proteinů. Metoda je testována na rostlinné beta glukozidáze Zm-p60.1, která je důležitá pro svou schopnost uvolňovat rostlinné hormony z glukokonjugátů.
Classification
Type
D - Article in proceedings
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/LC06034" target="_blank" >LC06034: Regulation of morphogenesis of plant cells and organs</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2007
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
MendelNet'07 Agro
ISBN
978-80-7375-119-7
ISSN
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e-ISSN
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Number of pages
1
Pages from-to
108-108
Publisher name
MZLU v Brno
Place of publication
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Event location
Brno
Event date
Jan 1, 2007
Type of event by nationality
CST - Celostátní akce
UT code for WoS article
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