Maize beta-glucosidase Zm-60.1 and its mutant forms: Optimization of purification procedure
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F07%3A00117765" target="_blank" >RIV/62156489:43210/07:00117765 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Maize beta-glucosidase Zm-60.1 and its mutant forms: Optimization of purification procedure
Original language description
beta-Glucosidases serve different roles in planta and indeed we find that plants have several different groups of this enzyme. Some of them can release active zeatin from zeatin-O-glucoside, a cytokinin conjugate thought to be the transport or storage form. One such enzyme is Zm-p60.1 from Zea mays. We have over-expressed the cDNA encoding Zm-p60.1 in E. coli and subsequently purified the recombinant protein using a purification procedure (Zouhar et al., 1999, Verdoucq et al., 2003) which uses a phosphate buffer system, imidazole as eluent. This procedure has been successfully applied to purify the native protein, but attempts to purify His-tagged mutants were not successful. The presence of glycerol (a protection agent) in the elution solutions causesprecipitation of some mutants, and protein denaturation occurred during hydrophobic column chromatography. We therefore had to completely revise the purification protocol. For purification of recombinant enzyme and its histidine mutant f
Czech name
Kukuřičná beta-glukosidasa Zm-p60.1 a jejích mutantních forem: Optimalisace purifikační procedury
Czech description
Práce řeší vylepšení purifikační rutiny isolace a purifikace rekombinantní kukuřičné beta-glukosidasy Zm-p60.1.
Classification
Type
D - Article in proceedings
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/LC06034" target="_blank" >LC06034: Regulation of morphogenesis of plant cells and organs</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2007
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
MendelNet'07 Agro
ISBN
978-80-7375-119-7
ISSN
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e-ISSN
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Number of pages
1
Pages from-to
103-103
Publisher name
MZLU v Brno
Place of publication
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Event location
Brno
Event date
Jan 1, 2007
Type of event by nationality
CST - Celostátní akce
UT code for WoS article
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