Studium vazby platinových cytostatik na bázi DNA struktury; Vliv glutathionu

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F07%3A00185066" target="_blank" >RIV/62156489:43210/07:00185066 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

čeština

Original language name

Study of binding of platinum based cytostatics to DNA structure; Influence of glutathione

Original language description

Although platinum based cytostatic drugs have been successfully used in the chemotherapy of cancer for more than 30 years, its biochemical mechanism of action is still unclear. The current accepted opinion about their mechanism of action is that the drugs induce their cytotoxic properties through binding to the nuclear DNA and subsequent interference with normal transcription, and/or DNA replication mechanisms. Nevertheless a cell has several mechanisms to prevent them from binding to the nuclear DNA. The main aim of this work was to investigate affecting of binding of cisplatin to the nuclear DNA by the presence of glutathione (GSH), which can be considered as one of the possible molecules connected with occurring of drug resistance. Primarily we attempted to study the interactions between cisplatin and GSH using flow injection analysis with electrochemical detection (FIA-ED). The detecton limit for GSH was 100 pg mL-1. It clearly follows from the results obtained that the optimized t

Czech name

Study of binding of platinum based cytostatics to DNA structure; Influence of glutathione

Czech description

Although platinum based cytostatic drugs have been successfully used in the chemotherapy of cancer for more than 30 years, its biochemical mechanism of action is still unclear. The current accepted opinion about their mechanism of action is that the drugs induce their cytotoxic properties through binding to the nuclear DNA and subsequent interference with normal transcription, and/or DNA replication mechanisms. Nevertheless a cell has several mechanisms to prevent them from binding to the nuclear DNA. The main aim of this work was to investigate affecting of binding of cisplatin to the nuclear DNA by the presence of glutathione (GSH), which can be considered as one of the possible molecules connected with occurring of drug resistance. Primarily we attempted to study the interactions between cisplatin and GSH using flow injection analysis with electrochemical detection (FIA-ED). The detecton limit for GSH was 100 pg mL-1. It clearly follows from the results obtained that the optimized t

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

<a href="/en/project/IAA401990701" target="_blank" >IAA401990701: Study of binding of platinum cytostatics to DNA structure; Influence of metallothionein</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2007

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Tumor Biology

ISSN

1010-4283

e-ISSN

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Volume of the periodical

28

Issue of the periodical within the volume

9

Country of publishing house

CH - SWITZERLAND

Number of pages

1

Pages from-to

123

UT code for WoS article

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EID of the result in the Scopus database

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