The Use of RAPD and AFLP Markers for Characterisation of Winter Barley Genotypes for Breeding to Fusarium Head Blight Resistance

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F08%3A00112236" target="_blank" >RIV/62156489:43210/08:00112236 - isvavai.cz</a>

Alternative codes found

RIV/25328859:_____/08:#0000385

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

The Use of RAPD and AFLP Markers for Characterisation of Winter Barley Genotypes for Breeding to Fusarium Head Blight Resistance

Original language description

The objectives of the study were to test diversity among winter barley breeding lines exhibiting various sensitivity to FHB and to find RAPD markers and AFLP markers that will distinguish between susceptible and moderately resistant genotypes. A test ofa set of winter barley genotypes artificially infected in field trials by fusaria was carried out. Based on the results from field and laboratory evaluation and deoxynivalenol (DON) content assessment, barley genotypes with different responses to FHB were selected. The genotypes were hybridized and doubled haploid (DH) lines were derived in F1 generation using the in vitro androgenesis method. Initial parental components and derived DH lines were tested for FHB infection and DON content. A set of parental genotypes of winter barley was tested with 80 RAPD markers. Based on analyses of 80 RAPD primers in a set of parental genotypes of winter barley, the primer H15 was selected that provides specific product of 650-bp size for moderately

Czech name

Využití RAPD a AFLP markeů pro charakterizaci genotypů ozimého ječmene pro šlechtění na rezistenci k FHB

Czech description

Předmětem studie bylo studium genetické diversity slechtitelského materiálu lišícího se svojí citlivostí k FHB. Byly použity AFLP a RAPD markery. Pomocí RAPD primeru H15 byl identifikován produkt o velikosti 650 bp umožňující rozlišit náchlné a středně rezistentní genotypy ječmene. Tento produkt byl detekován u některých DN linií, které byly odvozeny z křížení rodičovských genotypů s rozdílnou citlivostí. jako prespektivní DH linie byla vybrána DH linie 610 z křížení Br2611m x Duet.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/GA521%2F03%2F0938" target="_blank" >GA521/03/0938: A study of spring and winter malting barley resistance to Fusarium head blight and development of genotypes with improved resistance using molecular markers</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2008

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Cereal Research Communications

ISSN

0133-3720

e-ISSN

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Volume of the periodical

1

Issue of the periodical within the volume

36

Country of publishing house

HU - HUNGARY

Number of pages

10

Pages from-to

1-10

UT code for WoS article

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EID of the result in the Scopus database

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