All

What are you looking for?

All
Projects
Results
Organizations

Quick search

  • Projects supported by TA ČR
  • Excellent projects
  • Projects with the highest public support
  • Current projects

Smart search

  • That is how I find a specific +word
  • That is how I leave the -word out of the results
  • “That is how I can find the whole phrase”

Electroanalysis of cisplatin-glutathione and cisplatin-DNA interactions

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F08%3A00133454" target="_blank" >RIV/62156489:43210/08:00133454 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Electroanalysis of cisplatin-glutathione and cisplatin-DNA interactions

  • Original language description

    Due to high reactivity of sulfhydryl group of glutathione (GSH) it is not a surprise that GSH can influence a treatment of diseases including cancer. An investigation of interactions of GSH with heavy metal based cytostatics like cisplatin can be one ofthe possible ways of studying of reasons how GSH can interrupt the cancer treatment. To investigate GS--cisplatin and DNA--cisplatin interactions, we employed electroanalytical techniques. Primarily, we attempted to characterize reduced GSH and oxidizedGSSG glutathione with respect to reach the lowest detection limits. Under the most suitable conditions, the limits of GSH and GSSG were 100 pg/ml and 50 ng/ml, respectively. Then, the interactions between GSH and cisplatin have been investigated. The results obtained have been evaluated the formation of the complex by spectrometry. Besides that we studied DNA--cisplatin interaction. The average concentration of the drug bound toDNAwas estimated as 8.1 ng of cisplatin per 500 ng of DNA. F

  • Czech name

    Elektroanalýza komplexu cisplatiny s glutathionem a interakce cisplatiny s DNA

  • Czech description

    Kuli vysoké reaktivitě SH-skupin glutathionu není překvapující, že glutathion může ovlivňovat léčbu nemocí včetně onkologických. V práci jsme se elektrochemickými metodami pokusili charakterizovat GSH a oxidovaný glutathion s ohledem na dosažení nejnižších detekčních limitů. Za nejvhodnějších podmínek jsme dosáhli limitů GSH/GSSG - 100 pg / ml a 50 ng / ml, resp. Pak byla zkoumána interakce GSH a cisplatiny a DNA. Průměrné množstvé léčebné cisplatiny navázané na 500 ng DNA byla 8,1 ng. Elektrochemická detekce představuje alternativní methodu pro stanovení biologicky aktivních sloučenin. Zde jsme ukázaly různé elektrochemické techniky jako vhodné nástroje pro sledování interakce mezi thioly, cytostatik a DNA.

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    BO - Biophysics

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/IAA401990701" target="_blank" >IAA401990701: Study of binding of platinum cytostatics to DNA structure; Influence of metallothionein</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2008

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Toxicology Letters

  • ISSN

    0378-4274

  • e-ISSN

  • Volume of the periodical

    180

  • Issue of the periodical within the volume

    1

  • Country of publishing house

    NL - THE KINGDOM OF THE NETHERLANDS

  • Number of pages

    1

  • Pages from-to

  • UT code for WoS article

  • EID of the result in the Scopus database