Optimisation of qualitative and semi-quantitative detection of genetically modified crops by PCR

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F09%3A00144430" target="_blank" >RIV/62156489:43210/09:00144430 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Optimisation of qualitative and semi-quantitative detection of genetically modified crops by PCR

Original language description

For qualitative and semi-quantitative detection of genetically modified maize we used two maize genotypes from the company Monsanto; i.e. a MON810-based transgenic hybrid of Bt-maize and a genetically non-modified control (isogenic line of Bt-maize). Theseeds were the subject matter of a "material transfer agreement" between Monsanto Europe and MUAF in Brno. For molecular analysis the genomic DNA was isolated from cultivated seedlings in the stage of one leaf (10-day-old plants grown at a temperature of 20oC and light regime of 12 h light and 12 h darkness) using the isolation kit DNeasy Plant Mini Kit. The plants were cultivated and genomic DNA was isolated in rooms intended for handling GMO's. After isolation the DNA concentration in the sample wasdetermined fluorimetrically. We used primers and PCR programmes published by Greiner et al. (2005) and Hernandéz et al. (2005). When introducing PCR methods of detection of Bt-maize the first step was to optimise the protocol for the dete

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/1M06030" target="_blank" >1M06030: Functional genomics and proteomics for crop improvement</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta Universitatis agriculturae et silviculturae Mendelianae Brunensis : Acta of Mendel University of agriculture and forestry Brno = Acta Mendelovy zemědělské a lesnické univerzity v Brně

ISSN

1211-8516

e-ISSN

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Volume of the periodical

LVII

Issue of the periodical within the volume

5

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

6

Pages from-to

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UT code for WoS article

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EID of the result in the Scopus database

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