Study of metallothionein oxidation by using of chip capillary electrophoresis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F09%3A00152382" target="_blank" >RIV/62156489:43210/09:00152382 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Study of metallothionein oxidation by using of chip capillary electrophoresis

Original language description

Metallothionein (MT) is a low-molecular mass protein playing an essential role in homeostasis of heavy metals ions. We used a chip-based CE for quantitative study of MT oxidation. After oxidation of MT by H2O2 we observed marked decrease in peaks heightsand shift of peaks positions to higher molecular mass, which corresponded with the time of the oxidation. Moreover, we observed that the proportion of high-molecular forms of MT was markedly increased. The oxidative changes were successfully reversed byusing of reducing agent prior to electrophoresis. Our method can reveal the stability MT aggregates, of which biological role still remains unclear.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

<a href="/en/project/IAA401990701" target="_blank" >IAA401990701: Study of binding of platinum cytostatics to DNA structure; Influence of metallothionein</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Electrophoresis

ISSN

0173-0835

e-ISSN

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Volume of the periodical

30

Issue of the periodical within the volume

23

Country of publishing house

DE - GERMANY

Number of pages

5

Pages from-to

4029-4033

UT code for WoS article

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EID of the result in the Scopus database

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