Engineering the cytokinin-glucoside specificity of the maize beta-d-glucosidase Zm-p60.1 using site-directed random mutagenesis.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F12%3A00177633" target="_blank" >RIV/62156489:43210/12:00177633 - isvavai.cz</a>

Alternative codes found

RIV/68081707:_____/12:00440809

Result on the web

<a href="http://dx.doi.org/10.1016/j.phytochem.2011.10.008" target="_blank" >http://dx.doi.org/10.1016/j.phytochem.2011.10.008</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.phytochem.2011.10.008" target="_blank" >10.1016/j.phytochem.2011.10.008</a>

Result language

angličtina

Original language name

Engineering the cytokinin-glucoside specificity of the maize beta-d-glucosidase Zm-p60.1 using site-directed random mutagenesis.

Original language description

The maize beta-d-glucosidase Zm-p60.1 releases active cytokinins from their storage/transport forms, and its over-expression in tobacco disrupts zeatin metabolism. The role of the active-site microenvironment in fine-tuning Zm-p60.1 substrate specificityhas been explored, particularly in the W373K mutant, using site-directed random mutagenesis to investigate the influence of amino acid changes around the 373 position. Two triple (P372T/W373K/M376L and P372S/W373K/M376L) and three double mutants (P372T/W373K, P372S/W373K and W373K/M376L) were prepared. Their catalytic parameters with two artificial substrates show tight interdependence between substrate catalysis and protein structure. P372T/W373K/M376L exhibited the most significant effect on naturalsubstrate specificity: the ratio of hydrolysis of cis-zeatin-O-beta-d-glucopyranoside versus the trans-zeatin-O-beta-d-glucopyranoside shifted from 1.3 in wild-type to 9.4 in favor of the cis- isomer. The P372T and M376L mutations in P372

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Phytochemistry

ISSN

0031-9422

e-ISSN

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Volume of the periodical

74

Issue of the periodical within the volume

2

Country of publishing house

GB - UNITED KINGDOM

Number of pages

9

Pages from-to

40-48

UT code for WoS article

300815700004

EID of the result in the Scopus database

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