Determination of eight polycyclic aromatic hydrocarbons and in pea plants (Pisum sativum L.) extracts by high performance liquid chromatography with electrochemical detection

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F12%3A00183684" target="_blank" >RIV/62156489:43210/12:00183684 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14310/12:00057259 RIV/62157124:16270/12:43871402 RIV/62157124:16370/12:43871248 RIV/00216305:26620/12:PU99111
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Determination of eight polycyclic aromatic hydrocarbons and in pea plants (Pisum sativum L.) extracts by high performance liquid chromatography with electrochemical detection
Original language description
In the field of plant physiology, knowledge about polycyclic aromatic hydrocarbons (PAHs), mechanisms of actions are still missing and are limited despite the fact that induction of oxidative stress by several PAHs has been demonstrated. Primarily, highperformance liquid chromatography coupled with UV detector and high performance liquid chromatography coupled with electrochemical detector were optimized for detection and quantification of the following PAHs as fluoranthene, pyrene, benzo[a]pyrene, naphthalene, benzo[a]antracene, methylanthracene, triphenylene and coronene. The optimized conditions were as follows: mobile phase - A: acetic acid (50 mM) and B: methanol (100 %). Compounds were eluted by linearly increasing gradient: 0 - 6 min (70 % B),6 - 10 min (100 % B), 10 - 13 min (100 % B), 13 - 14 min (70 % B), 14 - 20 min (70 % B). Detection was performed at 275 nm (UV detector) and at 900 mV (electrochemical detector). One analysis lasted less than 20 minutes. Detection limits
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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