Accumulation of cadmium by transgenic tobacco plants (Nicotiana tabacum L.) carrying yeast metallothionein gene revealed by electrochemistry

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F12%3A00198107" target="_blank" >RIV/62156489:43210/12:00198107 - isvavai.cz</a>

Alternative codes found

RIV/00216305:26620/12:PU99110

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Accumulation of cadmium by transgenic tobacco plants (Nicotiana tabacum L.) carrying yeast metallothionein gene revealed by electrochemistry

Original language description

Phytoremediation of heavy metals includes phytoextraction and phytoaccumulation, i.e. accumulation of pollution from soils or water into the plants. Inorganic pollutants like heavy metals cannot be chemically degraded and thus it has to be accumulated inroots and leaves of the plants in non-toxic forms. In this study, the influence of cadmium on transgenic Nicotiana tabacum plants carrying yeast metallothionein was investigated. Tobacco plants were exposed to 0, 50, 100 and 250 uM CdCl2 for 96 hours. Growth parameters as well as changes in content of cysteine, oxidized/reduced glutathione, phytochelatins (PC2, PC3 and PC4) were monitored using high performance liquid chromatography with electrochemical detection. Moreover, we exploited differential pulse voltammetry Brdicka reaction for determination of metallothionein and verified the results obtained by dot blot assay. In addition, all results were compared with results obtained using non-transgenic tobacco plants. Our results indic

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CG - Electrochemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

International Journal of Electrochemical Science

ISSN

1452-3981

e-ISSN

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Volume of the periodical

7

Issue of the periodical within the volume

2

Country of publishing house

RS - THE REPUBLIC OF SERBIA

Number of pages

22

Pages from-to

886-907

UT code for WoS article

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EID of the result in the Scopus database

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