Using of paramagnetic microparticles and quantum dots for isolation and electrochemical detection of influenza viruses' specific nucleic acids

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F13%3A00193692" target="_blank" >RIV/62156489:43210/13:00193692 - isvavai.cz</a>

Alternative codes found

RIV/00216305:26620/13:PU103043

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Using of paramagnetic microparticles and quantum dots for isolation and electrochemical detection of influenza viruses' specific nucleic acids

Original language description

The aim of this study was to suggest isolation protocol based on paramagnetic microparticles and multicolour electrochemical detection of three various influenza oligonucleotides (H1N1 influenza subtype, H3N2 influenza subtype and H5N1 influenza subtype)modified with nanoparticles (NPs) called quantum dots (QDs), which are made from zinc, cadmium or lead. Particularly, we described hybridization assay based on paramagnetic microparticles (MPs) and coupled with detection of three different influenza derived oligonucleotides (ODNs) labelled with QDs. Hybridization efficiency between NPs conjugated anti-senses and target nucleic acid hybrids was optimized and evaluated by the electrochemical analysis. The highest response of ODN-SH-NPs was determined forthe hybridization temperature of 25 °C. Further, we tested detection of the complexes via peak coming from the presence of ODNs and/or from the presence of metal ions in QDs. Under the optimized conditions, LODs (3 S/N) based on the dete

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

International Journal of Electrochemical Science

ISSN

1452-3981

e-ISSN

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Volume of the periodical

8

Issue of the periodical within the volume

1

Country of publishing house

RS - THE REPUBLIC OF SERBIA

Number of pages

14

Pages from-to

689-702

UT code for WoS article

316562800054

EID of the result in the Scopus database

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