Genomic analyses of dihydroflavonol reductase gene in genotypes of common wheat (Triticum aestivum L.) with nonstandard coloured caryopses
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F13%3A00209872" target="_blank" >RIV/62156489:43210/13:00209872 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Genomic analyses of dihydroflavonol reductase gene in genotypes of common wheat (Triticum aestivum L.) with nonstandard coloured caryopses
Original language description
Anthocyanins are responsible for the coloration in shades of blue, purple and red of various body parts of plants. Wheat caryopses with nonstandard coloration, specifically genotypes UC66049 and Tschermaks Blaukörniger Sommerweizen with blue aleurone, ANK28B and Abyssinskaya arraseita with purple pericarp were used in the experiment. Genotype Novosibirskaya 67 was used as a standard because it does not synthesize any pigments. Total RNA was isolated from caryopses by the phenol-chloroform method and transcribed into cDNA. In dihydroflavonol reductase (DFR) gene sequences obtained from direct sequencing of PCR product were detected several indels and single nucleotide polymorphisms. The similarity among all sequences of analyzed genotypes and sequence obtained from National Center for Biotechnology Information (NCBI) ranged between 94.59 to 100 %. The gene expression of DFR in samples varied during maturation. For the control genotype Novosibirskaya 67 there was highest DFR gene express
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
MendelNet 2013 - Proceedings of International PhD Students Conference
ISBN
978-80-7375-908-7
ISSN
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e-ISSN
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Number of pages
5
Pages from-to
678-682
Publisher name
Mendel University in Brno, Zemedelska 1, 613 00 Brno, Czech Republic
Place of publication
Faculty of Agronomy
Event location
Mendelova univerzita v Brně
Event date
Nov 20, 2013
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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