Shoot regeneration from various explants of horse chestnut (Aesculus hippocastanum L.)
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F13%3A00212775" target="_blank" >RIV/62156489:43210/13:00212775 - isvavai.cz</a>
Alternative codes found
RIV/00027073:_____/13:#0001614
Result on the web
<a href="http://dx.doi.org/10.1016/j.scienta.2013.06.030" target="_blank" >http://dx.doi.org/10.1016/j.scienta.2013.06.030</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.scienta.2013.06.030" target="_blank" >10.1016/j.scienta.2013.06.030</a>
Alternative languages
Result language
angličtina
Original language name
Shoot regeneration from various explants of horse chestnut (Aesculus hippocastanum L.)
Original language description
A protocol is described for the adventitious shoot bud regeneration from leaf, stem and root explants in A. hippocastanum. Different medium (Heller, WPM, Rugini and DKW) and growth regulators [N6-benzyladenine (BA), thidiazuron (TDZ), and naphthaleneacetic acid (NAA)] at a range of various concentrations have been investigated for organogenesis. Nutrient content in the culture media had a significant effect on regeneration of new shoots in Aesculus. The highest number and length of shoots was achieved on Rugini and WPM medium with moderate nutrient content. Adventitious shoot formation was observed in all types of explants. Regeneration frequency and shoot number depended on explant type and growth regulators. The highest regeneration (100%) and shootbud number (9.68) were recorded in stem explants on medium with 2 mg l--1 BA + 0.1 mg l--1 NAA. Generally, TDZ has lower effect on shoot regeneration in stem and leaf explants compared to BA. The additions of NAA to the culture medium imp
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EI - Biotechnology and bionics
OECD FORD branch
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Result continuities
Project
<a href="/en/project/QH81101" target="_blank" >QH81101: Prevention of new outplantings of Aesculus hippocastanum clone resistant to Cameraria ohridella</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Scientia Horticulturae
ISSN
0304-4238
e-ISSN
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Volume of the periodical
161
Issue of the periodical within the volume
September 24
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
5
Pages from-to
223-227
UT code for WoS article
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EID of the result in the Scopus database
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