Ultrastructural energetic model of porcine oocytes with different meiotic competence
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F13%3A43908823" target="_blank" >RIV/62156489:43210/13:43908823 - isvavai.cz</a>
Result on the web
<a href="https://mnet.mendelu.cz/mendelnet2013/mnet_2013_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2013/mnet_2013_full.pdf</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Ultrastructural energetic model of porcine oocytes with different meiotic competence
Original language description
The principal objective of the study was to characterize energetic components of matured porcine oocytes with different meiotic competence. Lipid droplets (LDs), activity of mitochondria and adenosine triphosphate (ATP) content represent the constituentsimportant for cytoplasmic maturation of mammalian oocytes. This research provides detection of LDs, ATP content and rearrangement of mitochondria in matured porcine oocytes with different meiotic competence. In porcine oocytes mitochondria surround LDsforming so-called metabolic units. Meiotic competence of porcine oocytes is directly influenced by the size of follicle from which oocytes are derived. The meiotically higher competent (MHC) and less competent (MLC) oocytes were isolated separately frommedium (6-9 mm) and small follicles (<5 mm) respectively by aspiration and cutting of ovarian cortex. LDs were stained by molecular probe Nile red. To determine localization of the lipid droplets protein, oocytes were labeled with an anti
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
GI - Farm animal breeding and farm animal pedigree breeding
OECD FORD branch
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Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
MendelNet 2013: Proceedings of International PhD Students Conference
ISBN
978-80-7375-908-7
ISSN
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e-ISSN
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Number of pages
9
Pages from-to
238-246
Publisher name
Mendelova univerzita v Brně
Place of publication
Brno
Event location
Brno
Event date
Nov 20, 2013
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
000366464800041