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Ultrastructural energetic model of porcine oocytes with different meiotic competence

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F13%3A43908823" target="_blank" >RIV/62156489:43210/13:43908823 - isvavai.cz</a>

  • Result on the web

    <a href="https://mnet.mendelu.cz/mendelnet2013/mnet_2013_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2013/mnet_2013_full.pdf</a>

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Ultrastructural energetic model of porcine oocytes with different meiotic competence

  • Original language description

    The principal objective of the study was to characterize energetic components of matured porcine oocytes with different meiotic competence. Lipid droplets (LDs), activity of mitochondria and adenosine triphosphate (ATP) content represent the constituentsimportant for cytoplasmic maturation of mammalian oocytes. This research provides detection of LDs, ATP content and rearrangement of mitochondria in matured porcine oocytes with different meiotic competence. In porcine oocytes mitochondria surround LDsforming so-called metabolic units. Meiotic competence of porcine oocytes is directly influenced by the size of follicle from which oocytes are derived. The meiotically higher competent (MHC) and less competent (MLC) oocytes were isolated separately frommedium (6-9 mm) and small follicles (<5 mm) respectively by aspiration and cutting of ovarian cortex. LDs were stained by molecular probe Nile red. To determine localization of the lipid droplets protein, oocytes were labeled with an anti

  • Czech name

  • Czech description

Classification

  • Type

    D - Article in proceedings

  • CEP classification

    GI - Farm animal breeding and farm animal pedigree breeding

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2013

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Article name in the collection

    MendelNet 2013: Proceedings of International PhD Students Conference

  • ISBN

    978-80-7375-908-7

  • ISSN

  • e-ISSN

  • Number of pages

    9

  • Pages from-to

    238-246

  • Publisher name

    Mendelova univerzita v Brně

  • Place of publication

    Brno

  • Event location

    Brno

  • Event date

    Nov 20, 2013

  • Type of event by nationality

    WRD - Celosvětová akce

  • UT code for WoS article

    000366464800041