Carbohydrate analysis: From sample preparation to HPLC on different stationary phases coupled with evaporative light-scattering detection
Result description
After 20 years of development, evaporative light-scattering detection has become the mainstream choice for the detection of various classes of natural products. Evaporative light-scattering detection continues to grow in popularity as a "quasi-universal"technique due to the specificity of the detection method, which is based on the scattering of laser light from non-volatile analyte particles. It represents an attractive alternative compared to other types of detection, such as refractive index detection and/or ultraviolet detection. This review presents issues concerned with the separation of carbohydrates in plant materials by high-performance liquid chromatography and evaporative light-scattering detection, as well as the advantages and limitationsrelating to the evaporative light-scattering detection method. Additionally, an overview of possible evaporative light-scattering detection applications in the analysis of carbohydrates in natural products is presented.
Keywords
evaporative light-scattering detectionsaccharidesstationary phasehigh-performance liquid chromatography
The result's identifiers
Result code in IS VaVaI
Result on the web
http://onlinelibrary.wiley.com/doi/10.1002/jssc.201301089/full
DOI - Digital Object Identifier
Alternative languages
Result language
angličtina
Original language name
Carbohydrate analysis: From sample preparation to HPLC on different stationary phases coupled with evaporative light-scattering detection
Original language description
After 20 years of development, evaporative light-scattering detection has become the mainstream choice for the detection of various classes of natural products. Evaporative light-scattering detection continues to grow in popularity as a "quasi-universal"technique due to the specificity of the detection method, which is based on the scattering of laser light from non-volatile analyte particles. It represents an attractive alternative compared to other types of detection, such as refractive index detection and/or ultraviolet detection. This review presents issues concerned with the separation of carbohydrates in plant materials by high-performance liquid chromatography and evaporative light-scattering detection, as well as the advantages and limitationsrelating to the evaporative light-scattering detection method. Additionally, an overview of possible evaporative light-scattering detection applications in the analysis of carbohydrates in natural products is presented.
Czech name
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Czech description
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Classification
Type
Jx - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Separation Science
ISSN
1615-9306
e-ISSN
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Volume of the periodical
37
Issue of the periodical within the volume
4
Country of publishing house
DE - GERMANY
Number of pages
15
Pages from-to
323-337
UT code for WoS article
330756500001
EID of the result in the Scopus database
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Basic information
Result type
Jx - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP
CB - Analytical chemistry, separation
Year of implementation
2014