Paramagnetic Particles and PNA Probe for Automated Separation and Electrochemical Detection of Influenza
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F14%3A00230473" target="_blank" >RIV/62156489:43210/14:00230473 - isvavai.cz</a>
Alternative codes found
RIV/00216305:26620/14:PU112580
Result on the web
<a href="http://dx.doi.org/10.1007/s10337-014-2737-2" target="_blank" >http://dx.doi.org/10.1007/s10337-014-2737-2</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s10337-014-2737-2" target="_blank" >10.1007/s10337-014-2737-2</a>
Alternative languages
Result language
angličtina
Original language name
Paramagnetic Particles and PNA Probe for Automated Separation and Electrochemical Detection of Influenza
Original language description
Considerable efforts have been devoted to the development of rapid and sensitive methods allowing the detection of viral nucleic acid. We herein describe an assay for identification of a specific influenza sequence. The suggested method was based on isolation using paramagnetic particles coupled with electrochemical detection of isolated product. Peptide nucleic acid (PNA) was used as a probe for hybridization and identification of the influenza-derived specific sequence. The use of PNA can show numerous benefits: PNA probe is not degradable by enzymes and the duplex of PNA with RNA/DNA is more thermostable and more resistant to pH changes than DNA/DNA or RNA/RNA duplexes. This PNA probe assay can be applied as a magnetically guidable tool for detection of DNA/RNA samples under different conditions.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/ED1.1.00%2F02.0068" target="_blank" >ED1.1.00/02.0068: Central european institute of technology</a><br>
Continuities
O - Projekt operacniho programu
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Chromatographia
ISSN
0009-5893
e-ISSN
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Volume of the periodical
77
Issue of the periodical within the volume
21-22
Country of publishing house
DE - GERMANY
Number of pages
8
Pages from-to
1425-1432
UT code for WoS article
345004800003
EID of the result in the Scopus database
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