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Paramagnetic Particles and PNA Probe for Automated Separation and Electrochemical Detection of Influenza

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F14%3A00230473" target="_blank" >RIV/62156489:43210/14:00230473 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216305:26620/14:PU112580

  • Result on the web

    <a href="http://dx.doi.org/10.1007/s10337-014-2737-2" target="_blank" >http://dx.doi.org/10.1007/s10337-014-2737-2</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1007/s10337-014-2737-2" target="_blank" >10.1007/s10337-014-2737-2</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Paramagnetic Particles and PNA Probe for Automated Separation and Electrochemical Detection of Influenza

  • Original language description

    Considerable efforts have been devoted to the development of rapid and sensitive methods allowing the detection of viral nucleic acid. We herein describe an assay for identification of a specific influenza sequence. The suggested method was based on isolation using paramagnetic particles coupled with electrochemical detection of isolated product. Peptide nucleic acid (PNA) was used as a probe for hybridization and identification of the influenza-derived specific sequence. The use of PNA can show numerous benefits: PNA probe is not degradable by enzymes and the duplex of PNA with RNA/DNA is more thermostable and more resistant to pH changes than DNA/DNA or RNA/RNA duplexes. This PNA probe assay can be applied as a magnetically guidable tool for detection of DNA/RNA samples under different conditions.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CE - Biochemistry

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/ED1.1.00%2F02.0068" target="_blank" >ED1.1.00/02.0068: Central european institute of technology</a><br>

  • Continuities

    O - Projekt operacniho programu

Others

  • Publication year

    2014

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Chromatographia

  • ISSN

    0009-5893

  • e-ISSN

  • Volume of the periodical

    77

  • Issue of the periodical within the volume

    21-22

  • Country of publishing house

    DE - GERMANY

  • Number of pages

    8

  • Pages from-to

    1425-1432

  • UT code for WoS article

    345004800003

  • EID of the result in the Scopus database