Hyphenated Solid Phase Extraction/Supercritical Fluid Extraction Methods for Extraction of Phenolic Compounds from Algae

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F14%3A43908664" target="_blank" >RIV/62156489:43210/14:43908664 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Hyphenated Solid Phase Extraction/Supercritical Fluid Extraction Methods for Extraction of Phenolic Compounds from Algae

Original language description

Hyphenated SPE/SFE method was developed for the extraction of phenolic compounds from plant matrices (6 algae species). 20 ml of liquid solvent was injected to the SPE cartridge and extracted at following conditions: 40 MPa, 80 degrees C, restrictor 10.5cm x 50 mu m i.d. for 60 min into 20 ml of MeOH as a trapping solvent. Two different methods were developed for different SPE sorbents (OASIS MCX 3cc, Waters and ADVANTA Spe-ed, Applied Separations) to prove that the principles work at different conditions. Each sorbent needs different modifier: 400 mu L of 2M HAc in situ + 50 % MeOH / 5 % ammonia in ultra-pure water on-line is best for OASIS, ADVANTA needs 50 % MeOH and 5 % ether (v/v) on-line. SPE/SFE method has higher recovery, gives cleaner extracts, enables the use of liquid samples via SPE cartridge and higher precision was achieved (no restrictor plugging). Reversed phase liquid chromatography-electrospray mass spectrometry analysis was used for analyte determination: the chroma

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Current Analytical Chemistry

ISSN

1573-4110

e-ISSN

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Volume of the periodical

10

Issue of the periodical within the volume

1

Country of publishing house

AE - UNITED ARAB EMIRATES

Number of pages

13

Pages from-to

86-98

UT code for WoS article

000326245500007

EID of the result in the Scopus database

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