Construction of multiplex quantitative PCR for detection of streptococcal mastitis

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F14%3A43908802" target="_blank" >RIV/62156489:43210/14:43908802 - isvavai.cz</a>
Result on the web
<a href="https://mnet.mendelu.cz/mendelnet2014/mnet_2014_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2014/mnet_2014_full.pdf</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Construction of multiplex quantitative PCR for detection of streptococcal mastitis
Original language description
The objective of this study was to develop a multiplex quantitative PCR-based method for simultaneous detection of Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis in biological samples. The cfb gene for CAMP factor was usedfor detection of S. agalactiae. The cpn60 gene was used for detection of S. dysgalactiae and the plasminogen activator gene for S. uberis detection. PCR for S. dysgalactiae performed optimally, but reactions of S. agalactiae and S. uberis showed poor andno amplification respectively.
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
MendelNet 2014: Proceedings of International Ph.D. Students Conference
ISBN
978-80-7509-174-1
ISSN
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e-ISSN
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Number of pages
4
Pages from-to
512-515
Publisher name
Mendelova univerzita v Brně
Place of publication
Brno
Event location
Brno
Event date
Nov 19, 2014
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
000362518300098

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