Seed proteome analysis and proteome dynamics during seed germination

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F15%3A43906943" target="_blank" >RIV/62156489:43210/15:43906943 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Seed proteome analysis and proteome dynamics during seed germination
Original language description
Despite the huge progress that has been made in the last decade, the molecular mechanisms regulating seed germination and early seed development are far from being resolved. Induction of metabolic genes involved in germination starts around 12 hours after imbibition. Thus, most of the early events are mediated by molecules stored in the seed during maturation and are not accessible to transcriptomic analyses. Proteome analysis has been extensively employed in the past but the coverage of observed seed proteome is relatively low even in present-day high-impact studies. Here, we analysed proteome of two model species, Arabidopsis thaliana and barley (Hordeum vulgare). We employed several complementary approaches to increase the proteome coverage and build a library suitable for targeted protein quantitation. The combination of fractionations and an alternative MS/MS data processing significantly improved our detection limits. Our results indicate that the seed proteome coverage is limite
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
MendelNet 2015: Proceedings of International PhD Students Conference
ISBN
978-80-7509-363-9
ISSN
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e-ISSN
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Number of pages
4
Pages from-to
412-415
Publisher name
Mendelova univerzita v Brně
Place of publication
Brno
Event location
Brno
Event date
Nov 11, 2015
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
000366466100076

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