The effect of metal ions on Staphylococcus aureus revealed by biochemical and mass spectrometric analyses
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F15%3A43907656" target="_blank" >RIV/62156489:43210/15:43907656 - isvavai.cz</a>
Alternative codes found
RIV/00216305:26620/15:PU113298
Result on the web
<a href="http://www.sciencedirect.com/science/article/pii/S0944501314000895" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0944501314000895</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.micres.2014.08.003" target="_blank" >10.1016/j.micres.2014.08.003</a>
Alternative languages
Result language
angličtina
Original language name
The effect of metal ions on Staphylococcus aureus revealed by biochemical and mass spectrometric analyses
Original language description
In this study, we focused on the effect of heavy metal ions in resistant strains of gram-positive bacteria Staphylococcus aureus using biochemical methods and mass spectrometry. Five nitrate solutions of heavy metals (Ag+, Cu2+, Cd2+, Zn2+ and Pb2+) were used to create S. aureus resistant strains. Biochemical changes of resistant strains in comparison with the non-resistant control strain of S. aureus were observed by microbiological (measuring - growth curves and inhibition zones) and spectrophotometric methods (antioxidant activity and alaninaminotransferase, aspartateaminotransferase, alkaline phosphatase, gamma-glutamyltransferase activities). Mass spectrometry was employed for the qualitative analysis of the samples (changes in S. aureus protein composition) and for the identification of the strains database MALDI Biotyper was employed. Alterations, in terms of biochemical properties and protein composition, were observed in resistant strains compared to non-resistant control strain. Our results describe the possible option for the analysis of S. aureus resistant strains and may thus serve as a support for monitoring of changes in genetic information caused by the forming of resistance to heavy metals.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CG - Electrochemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/EE2.4.31.0023" target="_blank" >EE2.4.31.0023: Partner Network for Bionanotechnological and Metallomic Research</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Microbiological Research
ISSN
0944-5013
e-ISSN
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Volume of the periodical
170
Issue of the periodical within the volume
January
Country of publishing house
DE - GERMANY
Number of pages
10
Pages from-to
147-156
UT code for WoS article
000347502500017
EID of the result in the Scopus database
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